Information Package / Course Catalogue
General Biochemistry Laboratory I
Course Code: BYL355
Course Type: Required
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 0
Prt.: 0
Credit: 1
Lab: 2
ECTS: 3
Objectives of the Course

To gain the ability to learn and apply biochemical methods.

Course Content

To learn practically the glassware, tools and solution preparation methods used in the laboratory, as well as amino acid, protein and enzyme activity determination methods.

Name of Lecturer(s)
Prof. Kubilay METİN
Learning Outcomes
1.Learn and apply laboratory working rules and solution preparation techniques.
2.Learn and apply chromatographic methods
3.Learn and apply qualitative and quantitative determination methods of amino acids and proteins.
4.Learn and apply the working principle of the UV-VIS Spectrophotometry instrument.
5.Learn and apply the determination and kinetics of enzyme activity.
Recommended or Required Reading
1.Lehninger Principles of Biochemistry
2.Lecture notes
Weekly Detailed Course Contents
Week 1 - Laboratory
General analysis techniques
Week 2 - Laboratory
General analysis techniques
Week 3 - Laboratory
pH and buffer solutions
Week 4 - Laboratory
Paper chromatography
Week 5 - Laboratory
Qualitative determination of amino acid
Week 6 - Laboratory
Quantitative amino acid determinations
Week 7 - Laboratory
Qualitative determination of protein
Week 8 - Laboratory
Principles of spectrophotometry
Week 9 - Laboratory
Quantitative determination of protein
Week 10 - Laboratory
Quantitative determination of protein
Week 11 - Laboratory
Precipitation of proteins
Week 12 - Laboratory
Enzymes in living tissues
Week 13 - Laboratory
Factors affecting enzyme activity (Temperature-pH)
Week 14 - Laboratory
Factors affecting enzyme activity ( [S] and [E])
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Assignment23210
Laboratory142256
Midterm Examination1224
Final Examination1325
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
OÇ-1
3
5
4
5
3
3
3
5
4
4
1
OÇ-2
3
5
4
5
3
3
3
5
4
4
1
OÇ-3
3
5
4
5
3
3
3
5
4
4
1
OÇ-4
3
5
4
5
3
3
3
5
4
4
1
OÇ-5
3
5
4
5
3
3
3
5
4
4
1
Adnan Menderes University - Information Package / Course Catalogue
2026