Information Package / Course Catalogue
General Biochemistry Laboratory II
Course Code: BYL356
Course Type: Required
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 0
Prt.: 0
Credit: 1
Lab: 2
ECTS: 3
Objectives of the Course

To gain the ability to learn and apply biochemical methods.

Course Content

Application of qualitative and quantitative carbohydrate determinations, total lipid and fatty acid determination methods, electrophoretic techniques, protein electrophoresis (SDS-PAGE), gel filtration and ion-exchange chromatography methods.

Name of Lecturer(s)
Prof. Kubilay METİN
Learning Outcomes
1.Learn and apply qualitative and quantitative carbohydrate determinations.
2.Learn and apply the method of obtaining total lipid and total fatty acid from a living tissue.
3.Learn to conduct research and observation in the laboratory
4.Learn and apply electrophoretic techniques (SDS-PAGE)
5.Learn and apply thin layer chromatography
Recommended or Required Reading
1.Lecture notes
2.Lehninger's Principles of Biochemistry / David L. Nelson and Michael M. Cox
Weekly Detailed Course Contents
Week 1 - Laboratory
Qualitative determination of carbohydrates
Week 2 - Laboratory
Qualitative determination of carbohydrates
Week 3 - Laboratory
Quantitative determination of carbohydrate
Week 4 - Laboratory
Quantitative determination of carbohydrate
Week 5 - Laboratory
Purification of glycogen
Week 6 - Laboratory
Glycogen determination
Week 7 - Laboratory
Saponification
Week 8 - Laboratory
Determination of total lipid
Week 9 - Laboratory
Determination of total fatty acids
Week 10 - Laboratory
Microscopic examination of urine
Week 11 - Laboratory
Electrophoretic techniques
Week 12 - Laboratory
Protein electrophoresis
Week 13 - Laboratory
Protein electrophoresis
Week 14 - Laboratory
Thin Layer Chromatography
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Assignment23210
Laboratory142256
Midterm Examination1224
Final Examination1325
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
OÇ-1
3
5
4
5
3
3
3
5
4
4
1
OÇ-2
3
5
4
5
3
3
3
5
4
4
1
OÇ-3
3
5
4
5
3
3
3
5
4
4
1
OÇ-4
3
5
4
5
3
3
3
5
4
4
1
OÇ-5
3
5
4
5
3
3
3
5
4
4
1
Adnan Menderes University - Information Package / Course Catalogue
2026