Information Package / Course Catalogue - Adnan Menderes University

General Information Unit Staff Academic Information Programme Outcomes Course Structure Diagram Contact Information

Person Responsible for Information Package

Prof. Esin POYRAZOĞLU

Prof. Nazan ÜZÜM

Prof. Özkan EREN

Assoc. Prof. Fatma BURSALI

Lec. Derya ULUĞ

Microtechnics

Course Code	: BYL327
Course Type	: Area Elective
Couse Group	: First Cycle (Bachelor's Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 2
Prt.	: 2
Credit	: 3
Lab	: 0
ECTS	: 4

Objectives of the Course

Teaching microscopic examination methods.

Course Content

Introduction to microscope and microtome, smear preparation technique, bone preparation. Paraffin technique; detection solution selection, preparation and application, rules to be considered in the detection process, dehydration process, embedding and embedding errors, sectioning and points to be considered when sectioning, painting, selection of paint for the purpose, preparation of paint and paint set and different painting techniques, mounting process.

Name of Lecturer(s)

Prof. Yücel KOCA

Learning Outcomes

1.	Learns how to make preparations of cells, tissues and organs to be examined under the microscope.
2.	Understands laboratory studies and uses basic methods.
3.	Learns and applies different preparation and microscopic examination methods and understands their importance.
4.	Knows the method of examination after live and detection, and understands the difference between them.
5.	Knows the paraffin preparation method, applies it, and produces solutions to the problem.
6.	Examines and interprets the resulting preparation at the microscope level.
7.	He/she reaches a level where he/she can teach examination methods.
8.	Solve problems in laboratory studies and establish relationships with different fields during interpretation processes.

Recommended or Required Reading

1.	Bancroft, JD., Cook, HC. (1994). Manual of Histological Techniques and Their Diagnostic Application. London: Churchill Livingstone. 457 p. ISBN: 0-443-04534-8
2.	Prophet EB., Mills B., Arrington JB., Sobin LH. (1992). Laboratory Methods in Histotechnology. ISSN: 1-881041-00-X
3.	Demir R. (Editor), Histological Staining Techniques, Palme Publishing, Ankara 2001
4.	Internet - scientific web pages.

Weekly Detailed Course Contents

Week 1 - Theoretical & Practice

Introduction to the microscope and microtome.

Week 2 - Theoretical & Practice

Introduction of experimental animals, selection and care of experimental animals in experiments.Preparation of the experimental animal for the operation, dissection, tissue and organ harvesting.

Week 3 - Theoretical & Practice

The importance of fixation, selection of fixation solutions (fixatives). Preparation of fixatives.

Week 4 - Theoretical & Practice

Points to be considered during the detection process are detection errors. The process of detecting tissues.

Week 5 - Theoretical & Practice

Dehydration and blocking process, its purpose and importance, points to be considered in these processes. Dehydration process; passing fixed tissues through a graded alcohol series. Blocking; The process of embedding tissues in paraffin.

Week 6 - Theoretical & Practice

Introduction and working principles of microtome, explanation of microtome types. Introduction and use of the microtome used in the laboratory.

Week 7 - Theoretical & Practice

The importance of sectioning, points to consider when sectioning. Taking sections of fixed and blocked tissues with a microtome.

Week 8 - Theoretical & Practice

The importance of sectioning, points to consider when sectioning (continued). Taking sections of fixed and blocked tissues with a microtome (continued) (Midterm Exam)

Week 9 - Theoretical & Practice

Staining process; stain and staining methods (I) Preparation of stains and stain set.

Week 10 - Theoretical & Practice

Staining process; stains and staining methods (II) The process of staining the resulting sections.

Week 11 - Theoretical & Practice

Stains and staining methods(III) The process of staining the resulting sections (continued).

Week 12 - Theoretical & Practice

Explanation of the mounting process and points to be taken into consideration. Mounting the painted sections with a mounting medium and turning them into permanent preparations.

Week 13 - Theoretical & Practice

Process, importance and purpose of smear preparation. Application of the smear preparation technique.

Week 14 - Theoretical & Practice

Process, importance and purpose of bone preparation. Preparation of the bone preparation.

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%40
Final Examination	1	%60

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	1	2	42
Lecture - Practice	14	1	2	42
Individual Work	6	0	1	6
Midterm Examination	1	5	1	6
Final Examination	1	7	1	8
TOTAL WORKLOAD (hours)				104

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7	PÇ-8	PÇ-9	PÇ-10	PÇ-11	PÇ-12	PÇ-13	PÇ-14	PÇ-15
OÇ-1	5	5	5	5		5		5	4				5	4
OÇ-2	5	5	5	5		5		5	4				5	4
OÇ-3	5		5	5				5	4				5
OÇ-4	5	5	4	5				5	4				5
OÇ-5	5		3	5				5	4				5
OÇ-6	5		3	5				5	4				5
OÇ-7	5	5	4	5		5		5	4				5	4
OÇ-8	5		4	5		5		5	4				5	4