Information Package / Course Catalogue
| Course Code | : BYL433 |
| Course Type | : Area Elective |
| Couse Group | : First Cycle (Bachelor's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 2 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 4 |
To learn purification and characterization methods of proteins from tissues and cells.
Storage of tissues and cells, homogenization, centrifugation, precipitation of proteins, removal of salts, concentration of proteins, protein and enzyme determination, electrophoresis and chromatographic techniques and characterization methods of enzymes will be taught practically.
| Prof. Kubilay METİN |
| 1. | Learn and apply homogenization and centrifugation techniques |
| 2. | Learn and apply ultrafiltration and dialysis methods |
| 3. | Learn and apply column chromatography techniques |
| 4. | Learn and apply protein determination and purification steps |
| 5. | Explain and apply how enzyme activity determination and characterization are performed. |
| 1. | Protein purification protocols (Paul Cutler) |
| 2. | The protein protocols handbook (John M. Walker |
| 3. | Lecture notes |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %40 |
| Final Examination | 1 | %60 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 0 | 2 | 28 |
| Lecture - Practice | 14 | 2 | 2 | 56 |
| Assignment | 2 | 3 | 2 | 10 |
| Midterm Examination | 1 | 2 | 2 | 4 |
| Final Examination | 1 | 3 | 2 | 5 |
| TOTAL WORKLOAD (hours) | 103 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | PÇ-12 | PÇ-13 | PÇ-14 | PÇ-15 | |
OÇ-1 | 3 | 3 | 3 | 5 | 3 | 3 | 3 | 4 | 3 | 3 | 3 | ||||
OÇ-2 | 3 | 3 | 3 | 5 | 3 | 3 | 3 | 4 | 3 | 3 | 3 | ||||
OÇ-3 | 4 | 5 | 3 | 3 | 3 | 4 | 3 | 3 | |||||||
OÇ-4 | 3 | 5 | 3 | 3 | 4 | 3 | 3 | ||||||||
OÇ-5 | 4 | 4 | |||||||||||||