Information Package / Course Catalogue
Biochemistry Laboratory II
Course Code: KMY356
Course Type: Required
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 0
Prt.: 0
Credit: 2
Lab: 3
ECTS: 3
Objectives of the Course

Teaching methods for recognition and qualitative and quantitative analysis of biochemical molecules.

Course Content

Learning analysis techniques that can be applied to biochemical samples.

Name of Lecturer(s)
Lec. Rukiye YAVAŞER BONCOOĞLU
Learning Outcomes
1.Gain information about enzyme immobilization and purification.
2.Learns the principles and application of electrophoretic techniques.
3.Learns DNA and RNA isolation techniques.
4.Learns vitamin and metabolite analysis.
5.Learns the methods applied to measure enzyme activity.
6.Understands lipid and carbohydrate tests.
Recommended or Required Reading
1.Laboratory manual.
2.Biyokimya Cilt I, Prof. Dr. Engin M. Gözükara Nobel Kitabevi, 2011.
3.Experimental Biochemistry J.M. Clark,Jr and R.L. Switzer, W.H. Freeman and Company, 1977.
4.Biochemistry Laboratory Manual, F.M. Strong, G. H. Koch, Wm. C. Brown Company Publishers, 1981.
Weekly Detailed Course Contents
Week 1 - Laboratory
General information and preparation about the laboratory
Week 2 - Laboratory
Thin layer chromatography of amino acids
Week 3 - Laboratory
Calculation of saponification number in lipids
Week 4 - Laboratory
Determination of catalase by titration method
Week 5 - Laboratory
Determining the optimum properties of amylase
Week 6 - Laboratory
Determination of kinetic parameters of laccase enzyme
Week 7 - Laboratory
Usage of immobilized purple cabbage juice as a pH indicator
Week 8 - Laboratory
Topic Review (Midterm Examination)
Week 9 - Laboratory
RNA isolation from yeast
Week 10 - Laboratory
DNA isolation from kiwi
Week 11 - Laboratory
Crystallization of lactose from milk
Week 12 - Laboratory
Tollen's test for carbohydrates
Week 13 - Laboratory
Determination of reducing power of vitamin tablets
Week 14 - Laboratory
Electrophoresis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%12
Final Examination1%60
Quiz12%12
Report12%16
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Assignment121012
Laboratory140342
Quiz12006
Midterm Examination1516
Final Examination110111
TOTAL WORKLOAD (hours)77
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
OÇ-1
5
3
2
OÇ-2
5
3
3
OÇ-3
5
3
3
2
OÇ-4
5
3
2
OÇ-5
5
3
3
3
OÇ-6
5
3
4
2
Adnan Menderes University - Information Package / Course Catalogue
2026