Information Package / Course Catalogue
Methods in Molecular Biology
Course Code: ZBK555
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course of use of The methods used in molecular biology is to teach Biomolecules (nucleic asid RNA DNA,proteins) and isolation and analysis methods of the biomolecules.

Course Content

Brief of history of molecular biology, molecular organization of cells, biomoleculs, Nucleic asid, structural features of nucleic asid, chemical properties of nucleic asid , physical properties of nucleic asid. Furtermore the course covers the whole of general using methods on molecular biology.DNA and RNA isolation methods from the plant materials, agorose gel electrophoresis, PAGE (polyacrylamide gel electrophoresis ), Restiriction endonucleases, nucleic asid hybridization, protein isolation and analysis methods.

Name of Lecturer(s)
Lec. Sevdiye YORGANCI
Learning Outcomes
1.To be able to understand molecular structure of genetic material
2.İnformation about molecules in molecular biology.
3.Teaching several techniques that is used in Molecular Biology
4.
5.
Recommended or Required Reading
1.Erkan S. 1992, Moleküler Biyoloji Doğruluk Mat. İzmir 140s.
2.Dilsiz N. 2004 Moleküler Biyoloji Palme Yayıncılık, Ankara, 220s
3.Smith and Wood 1991,Molecular Biology and Biotechnology Chapman and Hall London 247s
4.Sambrook and Russell,(2001) Molecular Clonning, Cold Spring Harbor Laboratory Press, ISBN-0-87969-577-3
5.Temizkan G.,Arda N.(Ed.)(2004),Moleküler Biyolojide Kullanılan Yöntemler,Nobel Kitapevleri, ISBN975-420-347-4
Weekly Detailed Course Contents
Week 1 - Theoretical
History of molecular biology, relation with other science, biomolecules
Week 2 - Theoretical
Cell molecular organizations, cellular localization and roles of NA,
Week 3 - Theoretical
Nucleic asid, structural features of NA, Chemical properties of NA, physical properties of NA
Week 4 - Theoretical
DNA replication and repair mechanism, Recombinant DNA tecnology, protein, restiriksiyon enzymes
Week 5 - Theoretical
General methods on molecular biology lab.
Week 6 - Theoretical
DNA isolation and analysis, DNA transformation, Polimerase chain reaction RNA and
Week 7 - Theoretical
DNA isolation and analysis, DNA transformation, Polimerase chain reaction RNA and
Week 8 - Theoretical
RNA isolation and analysis
Week 9 - Theoretical
dsRNA izolation and analysis
Week 10 - Theoretical
Polimerase chain reaction
Week 11 - Theoretical
RNA/DNA hybridization
Week 12 - Theoretical
Bioinformatics
Week 13 - Theoretical
Gen cloning
Week 14 - Theoretical
Protein isolation and analysis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice143270
Midterm Examination134135
Final Examination138139
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
OÇ-1
4
4
3
5
5
4
OÇ-2
4
4
3
5
5
4
OÇ-3
4
4
3
5
5
4
OÇ-4
4
4
3
5
5
4
OÇ-5
4
4
3
5
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026