Information Package / Course Catalogue
Plant Cell and Tissue Culture
Course Code: BİO519
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

Teaching of alternative plant propagation techniques by using plant cell and tissue culture methods.

Course Content

Cell and tissue culture, micropropagation, organogenesis, somatic embryogenesis, callus culture, somaclonal variation, haploid plant production, the production of disease-free plant

Name of Lecturer(s)
Learning Outcomes
1.To be able to gain knowledge on basic concepts of plant cell and tissue culture
2.To be able to understand the basic lab techniques on tissue culture
3.To be able to evaluate data from studies on different applications of plant tissue culture
4.To be able to gain ability to discuss and prepare of presentation via homeworks and oral presentations given during the course
5.To be able to plan new researches to evaluate uses of global or local plant resources by alternative methods
Recommended or Required Reading
1.Plant Biotechnology Vol 1. Tissue Culture and its Applications Babaoğlu, M., Gürel, E., Özcan S.,S.Ü. Vakfı Yayınları, ISBN: 975-6652-04-7, (2001).
2.((Plant Biotechnology) vol.2. Genetic Engineering and its Applications) SBN: 975-6652-05-5, (2001).
Weekly Detailed Course Contents
Week 1 - Theoretical
Totipotency and differentiation
Week 1 - Practice
Stock solution and media preparation
Week 2 - Theoretical
cell and tissue culture
Week 2 - Practice
Stock solution and media preparation
Week 3 - Theoretical
Basic techniques
Week 3 - Practice
Stock solution and media preparation
Week 4 - Theoretical
organogenesis
Week 4 - Practice
Sterilization techniques
Week 5 - Theoretical
somatic embryogenesis
Week 5 - Practice
Removel of contamination
Week 6 - Theoretical
somaclonal variations
Week 6 - Practice
Organogenesis experiments
Week 7 - Theoretical
haploid plant production
Week 7 - Practice
organogenesis experiments
Week 8 - Theoretical
Disease-free plant production
Week 8 - Practice
micropropagation experiments
Week 9 - Theoretical
Protoplast culture ve somatic hybridization
Week 9 - Practice
micropropagation experiments
Week 10 - Theoretical
Protoplast culture ve somatic hybridization
Week 10 - Practice
Somatic embryogenesis experiments
Week 11 - Theoretical
micropropagation
Week 11 - Practice
Somatic embryogenesis experiments
Week 12 - Practice
midterm
Week 12 - Intermediate Exam
mid term
Week 13 - Theoretical
secondary metabolite production
Week 13 - Practice
callus culture
Week 14 - Theoretical
secondary metabolite production
Week 14 - Practice
callus culture
Week 15 - Theoretical
embryo culture
Week 15 - Practice
embryo culture
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice142256
Assignment142028
Reading140228
Midterm Examination112214
Final Examination112214
TOTAL WORKLOAD (hours)196
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
4
4
4
4
4
4
4
4
4
4
OÇ-2
3
3
3
3
3
3
3
3
3
3
OÇ-3
4
4
4
4
4
4
4
4
4
4
OÇ-4
3
3
3
3
3
3
3
3
3
3
OÇ-5
4
4
4
4
4
4
4
4
4
4
Adnan Menderes University - Information Package / Course Catalogue