Information Package / Course Catalogue
Introduction to Biotechnology in Field Crops
Course Code: ZTB540
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to defination of biotechnology-related terms, application areas in field crops, identification of biotechnology samples used in past and present, collection of analysis material, DNA and RNA isolations, expression of genes, gene transfer techniques, molecular marker methods and biotechnology on grain quality in abiotic stress conditions in field crops theoretical and practical training of research.

Course Content

Explain of information on important techniques and their use in field crops within the scope of plant biotechnology

Name of Lecturer(s)
Learning Outcomes
1.Investigation of plants at DNA and RNA levels
2.Gene expression analysis at RNA level
3.Explaining the molecular mechanisms of various agronomic properties
4.Abiotic stress resistant plant breeding activities in the light of this information
5.To be able to use appropriate genetic markers to be used in plant breeding
Recommended or Required Reading
1.C. Neal Stewart. PLANT BIOTECHNOLOGY AND GENETICS: Principles, Techniques, and Applications. 2008. Wiley. John Wiley & Sons, Inc., Hoboken, New Jersey.
2.Srivastava, P.S., Narula A., Srivastava S. Plant. 200. Biotechnology and Molecular Markers. Kluwer Academıc Publıshers New York, Boston, Dordrecht, London, Moscow. eBook ISBN: 1-4020-3213-7.
Weekly Detailed Course Contents
Week 1 - Theoretical
The definition of plant biotechnology, and the purposes and areas of use of biotechnology
Week 2 - Theoretical
Identification of structure of cell, chromosomes, DNA, RNA, gene, locus concepts
Week 3 - Theoretical
Structure of the gene and Gene expression analysis
Week 4 - Theoretical
Expression of genes, investigation of translation concepts
Week 5 - Theoretical
Explanation of protein biosynthesis
Week 6 - Theoretical
Introduction of DNA and RNA isolation, polymerase chain reaction (PCR)
Week 7 - Theoretical
Introduction of basic principles of gel electrophoresis
Week 8 - Intermediate Exam
Midterm exam
Week 9 - Theoretical
Visualization of products obtained as a result of molecular analysis and evaluation of results
Week 10 - Theoretical
Molecular biology and physiology of salinity stress
Week 11 - Theoretical
Molecular biology and physiology of drought stress
Week 12 - Theoretical
Molecular biology and physiology of temperature stress
Week 13 - Theoretical
Discussion of molecular studies on quality characteristics of protein and oil in the scope of grain quality in field crops
Week 14 - Theoretical
Developments in DNA markers and their use in field crops
Week 15 - Theoretical
Discussion of the current and future effects of genetically modified organisms
Week 16 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Lecture - Practice140228
Assignment50525
Term Project203060
Individual Work501050
Midterm Examination1246
Final Examination1224
TOTAL WORKLOAD (hours)201
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
4
4
5
5
5
4
5
5
5
5
OÇ-2
5
5
5
5
5
5
5
5
5
5
OÇ-3
5
5
5
5
5
5
5
5
5
5
OÇ-4
5
5
5
5
5
5
5
5
5
5
OÇ-5
5
4
5
4
5
4
5
4
5
4
Adnan Menderes University - Information Package / Course Catalogue