Information Package / Course Catalogue - Adnan Menderes University

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Recombinant DNA Technology

Course Code	: ZZO510
Course Type	: Area Elective
Couse Group	: Second Cycle (Master's Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 3
Prt.	: 0
Credit	: 3
Lab	: 0
ECTS	: 8

Objectives of the Course

It is the objective of this course to enable the undergraduate students to understand tools of manipulation for the DNA and RNA and methods of the recombinant DNA technology.

Course Content

The basic theory of recombinant DNA technologies, criteria of vector selection, the use of advanced analysis methods used in recombinant DNA technology

Name of Lecturer(s)

Assoc. Prof. Onur YILMAZ

Learning Outcomes

1.	To know DNA and RNA manipulation tools
2.	To learn recombinant DNA methods
3.	To know criteria of vector selection used in recombinant DNA technology
4.	To learn the use of advanced analysis methods used in recombinant DNA technology
5.	To learn transgenesis

Recommended or Required Reading

1.	Gene Cloning and DNA Analysis: An Introduction, 6th Edition, T. Brown, Wiley-Blackwell, ISBN: 978-1-4051-8173-0,
2.	From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, Dale, von Schantz, John Wiley & Sons, Ltd, ISBN: 978-0-470-01734-0, 2007
3.	Principles of Gene Manipulation and Genomics, 7th Edition, Primrose, Twyman, Blackwell Publishing, ISBN: 978-1-4051-3544-3, 2006

Weekly Detailed Course Contents

Week 1 - Theoretical

Definition of Recombinant DNA technology and genetic engineering and applications in the field

Week 2 - Theoretical

Basic genetic engineering techniques (solutions, agarose gel electrophoresis, Southern, Northern, Western Blot, etc.)

Week 3 - Theoretical

RNA and DNA modification enzymes (restriction endonucleases, ligase, kinase, phosphatases, polymerases, nucleases, etc.)

Week 4 - Theoretical

Cloning and expression vectors

Week 5 - Theoretical

Cloning and expression vectors

Week 6 - Theoretical

Gene Transfer Methods (transformation, electroporation, conjugation, etc.)

Week 7 - Theoretical

Gene Transfer Methods (transformation, electroporation, conjugation, etc.)

Week 8 - Theoretical

Polymerase Chain Reaction for manipulation of gene (Special methods of PCR, primer design for cloning)

Week 9 - Theoretical

Gene Cloning and Special Strategies

Week 10 - Theoretical

Selection of recombinant , screening and confirmation methods (Sequence Analysis, digest with restriction enzymes, PCR)

Week 11 - Theoretical

Protein Production with cloned gene (Types of expression vectors and features)

Week 12 - Theoretical

Protein Production with cloned gene (Types of expression vectors and features)

Week 13 - Theoretical

Effects of Recombinant DNA technology on the biological sciences and biotechnology

Week 14 - Theoretical

Effects of Genetic engineering on the biological sciences and biotechnology

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%30
Final Examination	1	%70

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	2	4	84
Assignment	9	8	1	81
Midterm Examination	1	14	1	15
Final Examination	1	14	1	15
TOTAL WORKLOAD (hours)				195

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7
OÇ-1	5
OÇ-2		5	3
OÇ-3		5	3
OÇ-4	5	5	5	5
OÇ-5	5	5	5

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