Information Package / Course Catalogue - Adnan Menderes University

General Information Unit Staff Academic Information Programme Outcomes Course Structure Diagram Contact Information

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Application Fields of Chromatography in Biochemistry

Course Code	: VBY538
Course Type	: Area Elective
Couse Group	: Second Cycle (Master's Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 2
Prt.	: 2
Credit	: 3
Lab	: 0
ECTS	: 3

Objectives of the Course

Theory of chromatography, definition and general principle of chromatography, classification of chromatography, thinlayer chromatography, colon chromatography, paper chromatography, high performance liquid chromatography, gas chromatography, general detection methods.

Course Content

Name of Lecturer(s)

Lec. Gamze Sevri EKREN AŞICI

Learning Outcomes

1.	To be able to comprehend a general knowledge about cromatography
2.	To be able to explain the principles of chromatography
3.	To be able to comprehend knowledge about thinlayer chromatography
4.	To be able to comprehend knowledge about High performance liquid chromatography
5.	To be able to comprehend knowledge about gas chromatography
6.	To be able to use the material learned effectively.

Recommended or Required Reading

1.	Biyokimya Güneş Tıp Kitapevi
2.	Biyokimya Leninger
3.	Biyokimya Lipinkot

Weekly Detailed Course Contents

Week 1 - Theoretical

Theory of chromatography, definitions and general principles

Week 1 - Practice

Introduction of main laboratory

Week 2 - Theoretical

Classification of chromatography

Week 2 - Practice

Learning about the devices

Week 3 - Theoretical

Technical features

Week 3 - Practice

Preparation of application plan

Week 4 - Theoretical

Sources of errors, advantages and disadvantages

Week 4 - Practice

Preparation of the necessary equipment and devices

Week 5 - Theoretical

Application areas

Week 5 - Practice

Preparation of colones

Week 6 - Theoretical

Midterm exam

Week 6 - Practice

Application

Week 7 - Theoretical

Gel filtration chromatography

Week 7 - Practice

Application

Week 8 - Theoretical

affinity chromatography

Week 8 - Practice

Introduction to HPLC

Week 9 - Theoretical

Ion-exchange chromatography

Week 9 - Practice

Preliminary preparation for HPLC

Week 10 - Theoretical

Gas chromatography

Week 10 - Practice

Calibration

Week 11 - Practice

Application

Week 11 - Intermediate Exam

Midterm exam

Week 12 - Theoretical

High Performance Liquid Chromatography

Week 12 - Practice

Application

Week 13 - Theoretical

High Performance Liquid Chromatography

Week 13 - Practice

Evaluation of results

Week 14 - Theoretical

Usage in researchs

Week 14 - Practice

Discussion

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Final Examination	1	%100

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	1	2	42
Lecture - Practice	14	0	2	28
Midterm Examination	1	4	1	5
Final Examination	1	3	1	4
TOTAL WORKLOAD (hours)				79

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7	PÇ-8	PÇ-9	PÇ-10	PÇ-11	PÇ-12	PÇ-13	PÇ-14	PÇ-15
OÇ-1		4	4	4				3			3				3
OÇ-2		4	4	4				3			3				3
OÇ-3		4	4	4				3			3				3
OÇ-4				3			3			3
OÇ-5					3			3			3	3	3
OÇ-6													3	3	3

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