Information Package / Course Catalogue
Histochemical Methods
Course Code: VHE504
Course Type: Required
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 5
Objectives of the Course

To describe the purposes for histochemical and cytochemical techniques, to teach the basic principles of histochemistry and cytochemistry, histochemical methods also give examples.

Course Content

Basic principles of histochemistry and cytochemistry, the usage purposes of histochemical and cytochemical techniques, the examples of histochemical methods.

Name of Lecturer(s)
Prof. Ülker EREN
Learning Outcomes
1.Knows the usage areas of histochemical techniques
2.Knows the usage areas of cytochemical techniques
3. Comprehend basic principles of histochemistry.
4.Comprehend basic principles of cytochemistry.
5.Gain experience by making the sample histochemical staining.
Recommended or Required Reading
1.Banks WJ. (1986) Applied Veterinary Histology,Williams& Wilkins, USA.
2. Culling C.F.A., Allison R.T., Barr W.T.: Cellular Pathology Technique. Butterworths and Co Ltd, 1985, London.
3.Dellman HD, Brown LM. (1987) Textbook of Veterinary Histology, Lea&Febiger, USA
4.Gartner LP, Hiatt JL. (1997) Color Textbook of Histology, W.B. Saunders Company,USA.
5.Junqueira LC, Carneiro J. (1983) Basic Histology,The McGraw-Hill Companies,USA
6.Leeson RR, Leeson TS, Paparo AA. (1985) Textbook of Histology, W.B. Saunders Company. USA
7.Ross MH, Reith EJ, Romrell LJ. (1989) Histology. A Text and Atlas, Williams &Wilkins, London
Weekly Detailed Course Contents
Week 1 - Theoretical
Basic histochemical principles
Week 1 - Practice
Basic histochemical principles
Week 2 - Theoretical
Ions
Week 2 - Practice
Ions
Week 3 - Theoretical
Phosphates
Week 3 - Practice
Phosphates
Week 4 - Theoretical
Nucleic acids
Week 4 - Practice
Nucleic acids
Week 5 - Theoretical
Fulgen reaction
Week 5 - Practice
Fulgen reaction
Week 6 - Theoretical
Methyl green pyronin staining
Week 6 - Practice
Methyl green pyronin staining
Week 7 - Theoretical
Literature discussion
Week 7 - Practice
Literature discussion
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Demonstration of proteins
Week 9 - Practice
Millon reaction
Week 10 - Theoretical
Demonstration of glycogen
Week 10 - Practice
PAS reaction
Week 11 - Theoretical
Demonstration of glycogen
Week 11 - Practice
Best’s carmine staining method
Week 12 - Theoretical
Demonstration of lipids
Week 12 - Practice
Sudan black B staining method
Week 13 - Theoretical
Demonstration of lipids
Week 13 - Practice
Oil red O staining method
Week 14 - Theoretical
Demonstration of mucus
Week 14 - Practice
Alcian blue staining method ;Aldehyde fuchsin staining method
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130226
Lecture - Practice130226
Reading120224
Midterm Examination122224
Final Examination124125
TOTAL WORKLOAD (hours)125
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
5
4
4
OÇ-2
OÇ-3
OÇ-4
5
4
4
OÇ-5
5
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026