Information Package / Course Catalogue
Streptoavidin-Biotin Peroxidase Method
Course Code: VHE535
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The aim of course is to teach application of streptoavidin-biotin peroxidase method in paraffin and frozen sections: fixation, protection of antigen, dehydration and cleaning, problems of staining methods and its solving.

Course Content

Application of streptoavidin-biotin peroxidase method in paraffin and frozen sections: Fixation, protection of antigen, dehydration and cleaning, problems of staining methods and its solving.

Name of Lecturer(s)
Prof. Ülker EREN
Learning Outcomes
1.The student learns tissue fixation for streptoavidin-biotin peroxidase method.
2.The student learns protection of the antigen in this method.
3.The student learns tissue dehydration and clearing in this method.
4.The student learns staining procedure in this method.
5.Understands the staining procedure in this method.
Recommended or Required Reading
1.Polak, J.M., Van Noorden, S. (2003) Introduction to Immunocytochemistry, Garland Science London
2.Burry, R.W. (2010) Immunocytochemistry, Springer USA.
Weekly Detailed Course Contents
Week 1 - Theoretical
Streptoavidin-biotin peroxidase method
Week 1 - Practice
Identification of laboratory material for this method
Week 2 - Theoretical
Streptoavidin-biotin peroxidase method
Week 2 - Practice
Identification of laboratory material for this method
Week 3 - Theoretical
Purpose of the streptoavidin-biotin peroxidase method
Week 3 - Practice
Prepare of other solutions for this method
Week 4 - Theoretical
Fixatives for this method
Week 4 - Practice
Fixatives for this method
Week 5 - Theoretical
Fixatives for this method
Week 5 - Practice
Fixatives for this method
Week 6 - Theoretical
Prepare of fixatives for this method
Week 6 - Practice
Prepare of fixatives for this method
Week 7 - Theoretical
Prepare of fixatives for this method
Week 7 - Practice
Prepare of fixatives for this method
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Protection of the antigen in this method.
Week 9 - Practice
Protection of the antigen in this method.
Week 10 - Theoretical
Tissue dehydration and clearing in this method.
Week 10 - Practice
Tissue dehydration and clearing in this method.
Week 11 - Theoretical
Staining procedure in this method.
Week 11 - Practice
Staining procedure in this method.
Week 12 - Theoretical
The problems possible in this method and its solving.
Week 12 - Practice
Staining procedure in this method.
Week 13 - Theoretical
The problems possible in this method and its solving.
Week 13 - Practice
The problems possible in this method and its solving.
Week 14 - Theoretical
The problems possible in this method and its solving, Article discussion
Week 14 - Practice
The problems possible in this method and its solving, Article presentation
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130113
Lecture - Practice130226
Midterm Examination116117
Final Examination118119
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
4
2
4
4
4
OÇ-2
4
2
4
4
4
OÇ-3
4
2
4
4
4
OÇ-4
4
2
4
4
4
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026