Information Package / Course Catalogue
Basic Laboratory Principles and Methods of Stem Cell
Course Code: KHÜ503
Course Type: Required
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to provide students with a comprehensive perspective on basic and applied research for stem cells (basic and clinical), to provide students with the ability to interpret multidisciplinary research methods in relation to the general stem cell knowledge of students.

Course Content

Basic laboratory methods, laboratory preparation steps, stem cell culture, molecular methods related to stem cells are explained.

Name of Lecturer(s)
Lec. Yusuf Ziya ARAL
Prof. İrfan YAVAŞOĞLU
Learning Outcomes
1.Explain basic laboratory methods and principles
2.Examine preparation stages in stem cell research
3.Explain stem cell culture theoretically and practically
4.Examine molecular methods in stem cell research
5.Examine the use of cell culture in stem cell research
Recommended or Required Reading
1.Sell S, Stem Cells Handbook, Humana Press, second edition, 2013
2.Alberio R, Epiblast Stem Cells, Humana Press, Methods and protocols,Wiley Blackwell, 2013
3.Regad T, Sayers TJ, Rees R, Principle of Stem Cell Biology and Cancer,
4.Healy L, Ruban L, Atlas of Human Pluripotent Stem Cells in Culture, Springer, 2015
5.Rich IN, Stem Cell Protocols, Humana Press, 2015
Weekly Detailed Course Contents
Week 1 - Theoretical
Course description and general concepts
Week 2 - Theoretical
Stem Cell Research Laboratories and Infrastructure
Week 3 - Theoretical
Preparation steps in stem cell research (sample preparation, purification, spectral techniques, immune techniques,)
Week 4 - Theoretical
Preparation steps in stem cell research (electrophoresis, blotting)
Week 5 - Theoretical
Preparation steps in stem cell research (hybridization, microscopy)
Week 6 - Theoretical
Cell isolation techniques
Week 7 - Theoretical
DNA, RNA and Protein Isolations in Stem Cell Research
Week 8 - Intermediate Exam
Mid-term exam
Week 9 - Theoretical
PCR in Stem Cell Research
Week 10 - Theoretical
Epigenetic methods in stem cell research
Week 11 - Theoretical
Special staining and imaging techniques in stem cell research
Week 12 - Theoretical
Cell culture in stem cell research (basic techniques) -I
Week 13 - Theoretical
Cell culture in stem cell research (basic techniques) -II
Week 14 - Theoretical
Cellular treatments
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%20
Final Examination1%60
Assignment1%20
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory131239
Assignment220244
Laboratory131239
Midterm Examination124226
Final Examination148250
TOTAL WORKLOAD (hours)198
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
4
4
3
4
4
2
3
2
2
OÇ-2
3
5
3
3
4
2
3
2
OÇ-3
3
5
3
3
4
2
3
2
OÇ-4
4
4
3
4
4
2
5
2
OÇ-5
4
4
3
4
4
2
5
2
Adnan Menderes University - Information Package / Course Catalogue