Information Package / Course Catalogue
Stem Cell-spesific Immunohistochemistry and Imaging Methods
Course Code: KHÜ532
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 6
Objectives of the Course

The aim of this course is to teach immunofluorescence-immunohistochemistry methods used in stem cell studies and to explain imaging methods for stem cell tracking in the clinic

Course Content

Microscopes used in stem cell studies, immunofluorescence imaging, marking and imaging methods used to follow stem cells in the body after clinical use will be explained.

Name of Lecturer(s)
Learning Outcomes
1.Knows immunofluorescence-immunohistochemistry methods used in stem cell studies
2.Explain in vivo imaging methods used in root studies
3.Examine the imaging methods used in the follow-up after stem cell applications.
4.Knows molecular imaging in stem cells
5.Understand stem cell imaging in clinical studies
Recommended or Required Reading
1.Sell S, Stem Cells Handbook, Humana Press, second edition, 2013
2.Alberio R, Epiblast Stem Cells, Humana Press, Methods and protocols,Wiley Blackwell, 2013
3.Regad T, Sayers TJ, Rees R, Principle of Stem Cell Biology and Cancer,
4.Healy L, Ruban L, Atlas of Human Pluripotent Stem Cells in Culture, Springer, 2015
5.Rich IN, Stem Cell Protocols, Humana Press, 2015
Weekly Detailed Course Contents
Week 1 - Theoretical
Course description and general information
Week 2 - Theoretical
Types of microscopes
Week 3 - Theoretical
Immunofluorescence staining
Week 4 - Theoretical
Immunohistochemical staining
Week 5 - Theoretical
Pluripotent stem cell-specific immunohistochemistry
Week 6 - Theoretical
Mesenchymal stem cell-specific immunohistochemistry
Week 7 - Theoretical
Immunohistochemistry in cancer stem cells
Week 8 - Intermediate Exam
Mid-term exam
Week 9 - Theoretical
Molecular imaging in stem cells
Week 10 - Theoretical
Stem cell marking
Week 11 - Theoretical
In vivo stem cell tracking
Week 12 - Theoretical
Stem cell imaging in clinical trials
Week 13 - Theoretical
Review of articles-I
Week 14 - Theoretical
Review of articles-II
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%20
Final Examination1%60
Assignment1%20
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory131239
Assignment218138
Midterm Examination124226
Final Examination148250
TOTAL WORKLOAD (hours)153
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
3
3
4
3
5
4
3
3
OÇ-2
3
3
4
3
5
4
3
3
OÇ-3
3
3
4
3
5
4
3
3
OÇ-4
3
3
4
3
5
4
3
3
OÇ-5
3
3
4
3
5
4
3
3
Adnan Menderes University - Information Package / Course Catalogue