Information Package / Course Catalogue
Applied Cell Culture
Course Code: MBTK545
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

To give students information on basic and applied studies of cell cultures used in research studies.

Course Content

The basic principles of cell and tissue culture, designing the cell culture laboratory and the specific devices and materials, safety in cell culture laboratory, working principles in tissue culture laboratories, contamination, sterilization techniques, culture media and solutions, cell and tissue culture methods, primary cell culture, the culture of special cell line, teaching the application field of cell culture methods in molecular biology and recombinant DNA technology.

Name of Lecturer(s)
Assoc. Prof. Olcay BOYACIOĞLU
Learning Outcomes
1.1. Having knowledge about cell culture studies, and cell lines and cell banks,
2.2. Recognizing the work conditions and using and materials
3.3. Having knowledge about routine cell culture processes
4.4. Having knowledge about in vitro digestion methods
5.5. Having knowledge about cell viability assays.
Recommended or Required Reading
1.1. Basic Cell Culture, Second Edition, J. M. Davis, Oxford University Press, 2002.
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Introduction, cell culture types
Week 2 - Theoretical & Practice
Developing primary cell line
Week 3 - Theoretical & Practice
Immortal cell lines
Week 4 - Theoretical & Practice
Cell source and cell banks
Week 5 - Theoretical & Practice
The design of the cell culture laboratory conditions and the working environment
Week 6 - Theoretical & Practice
The selection of consumables used
Week 7 - Theoretical & Practice
The selection of media and other materials used
Week 8 - Theoretical & Practice
Culture techniques: Cell morphology (Midterm exam)
Week 9 - Theoretical & Practice
In vitro digestion methods
Week 10 - Theoretical & Practice
Methods to determine cell viability
Week 11 - Theoretical & Practice
Methods to determine cell viability
Week 12 - Theoretical & Practice
Determination of cell mortality
Week 13 - Theoretical & Practice
Specialized cell cultures-I
Week 14 - Theoretical & Practice
Specialized cell cultures-II
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%20
Final Examination1%40
Practice7%20
Quiz4%10
Attending Lectures1%10
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Lecture - Practice140228
Assignment2024
Reading140228
Individual Work140342
Quiz4028
Midterm Examination130131
Final Examination130131
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
OÇ-1
5
3
4
5
2
OÇ-2
3
5
2
1
1
1
1
OÇ-3
1
1
5
4
3
3
3
2
1
1
1
1
1
OÇ-4
1
2
2
5
1
OÇ-5
1
1
1
1
2
5
5
3
Adnan Menderes University - Information Package / Course Catalogue
2026