Information Package / Course Catalogue
Microscopic Technique
Course Code: VHE503
Course Type: Required
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 5
Objectives of the Course

To teach the preparation of tissues for light microscopy.

Course Content

Taking of tissue samples for light microscopy, tissue fixation, the chemical properties of fixatives, dehydration, clearing, infiltration, blocking of the tissues, sectioning and staining..

Name of Lecturer(s)
Lec. Özay GÜLEŞ
Prof. Şadiye KUM
Learning Outcomes
1.tudents learn the tissue sampling.
2.Students learn the blocking.
3.Student knows sectioning
4.Students comprehend the properties of staining procedure.
5. Students learn application staning methods.
Recommended or Required Reading
1.Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. (1989) Molecular Biology of the Cell, Garland Publishing, Inc. London.
2.Banks, W.J. (1986). Applied Veterinary Histology, Williams&Wilkins, U.S.A.
3.Culling C.F.A., Allison R.T., Barr W.T.: Cellular Pathology Technique. Butterworths and Co Ltd, 1985, London.
4.Eren Ü (1998) Mikroskop Bilgisi. (Yardımcı Ders Kitabı), ADÜ Basımevi, Aydın
5.Junqueira LC, Carneiro J. (2005) Basic Histology,The McGraw-Hill Companies,USA
6.Kierszenbaum, A. L. (2007) Histology and Cell Biology. An introduction to Pathology, Mosby, Elsevier, Kanada.
7.Ross MH, Reith EJ, Romrell LJ. (1989) Histology. A Text and Atlas, Williams &Wilkins, London
Weekly Detailed Course Contents
Week 1 - Theoretical
Tissue sampling for light microscopy
Week 1 - Practice
Tissue sampling for light microscopy
Week 2 - Theoretical
Fixation and fixatives
Week 2 - Practice
Fixation of tissue samples
Week 3 - Theoretical
Properties of chemical fixatives
Week 3 - Practice
Examples of mixture fixatives
Week 4 - Theoretical
Properties of chemical fixatives
Week 4 - Practice
Examples of mixture fixatives
Week 5 - Theoretical
Dehidration
Week 5 - Practice
Dehidration
Week 6 - Theoretical
Clearing
Week 6 - Practice
Clearing
Week 7 - Theoretical
Protection from chemical hazards
Week 7 - Practice
Protection from chemical hazards
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Infiltration
Week 9 - Practice
Infiltration
Week 10 - Theoretical
Tissue blocking
Week 10 - Practice
Tissue blocking
Week 11 - Theoretical
Tissue blocking
Week 11 - Practice
Tissue blocking
Week 12 - Theoretical
Tissue sectioning
Week 12 - Practice
Tissue sectioning
Week 13 - Theoretical
Tissue sectioning
Week 13 - Practice
Tissue sectioning
Week 14 - Theoretical
Tissue staining
Week 14 - Practice
Tissue staining
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130113
Lecture - Practice130226
Laboratory130226
Reading90218
Midterm Examination119120
Final Examination120222
TOTAL WORKLOAD (hours)125
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
5
2
4
4
3
4
4
4
4
4
4
4
OÇ-2
5
2
4
4
3
4
4
4
4
4
4
4
OÇ-3
5
2
4
4
3
4
4
4
4
4
4
4
OÇ-4
5
2
4
4
3
4
4
4
4
4
4
4
OÇ-5
5
2
4
4
3
4
4
4
4
4
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026