Information Package / Course Catalogue
Advanced Techniques in Molecular Biology
Course Code: ZBY510
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

To teach of aim and principles of the molecular biology techniques, to develope skills of student about selection and application of the proper techniques for certain aim, to give them experience and perspective about interpret of the results .

Course Content

The basic techniques for diverse molecular biology analysis; isolation, purification and seperation of the biomolecules (DNA, RNA and proteins) and their further analysis.

Name of Lecturer(s)
Assoc. Prof. Emre SEVİNDİK
Learning Outcomes
1.To be educated about molecular biology analysis
2.To be educated about principles of the working in molecular biology laboratory
3.To be educated about application of the basic and advanced molecular biolgy techniques
4.To be educated about interpretion of the results
5.Learn about replication and gene expression
Recommended or Required Reading
1.Güler Temizkan and Nazlı Arda, Methods Used in Molecular Biology, Nobel Tıp Kitapevi, İstanbul, 2007 (ISBN: 9789754205831)
2.Atilla Özalpan and Narçin P. Ünsal, Genomic Applications, T.C. İstanbul Kültür University Publications, Istanbul, 2008
3.Dale, von Schantz, From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, John Wiley & Sons, Ltd, USA, 2007 (ISBN: 9780470017340)
Weekly Detailed Course Contents
Week 1 - Theoretical
Biosafety in molecular biology laboratory and common work rules
Week 2 - Theoretical
Fundamental principles of isolation and seperation of biomacromolecules
Week 3 - Theoretical
DNA isolation, qualitative and quantitative analysis of DNA
Week 4 - Theoretical
DNA seperation and visualisation methods
Week 5 - Theoretical
Plasmid DNA and vectors
Week 6 - Theoretical
Cutting of DNA by restriction endonucleases and ligation
Week 7 - Theoretical
Bacterial cloning od DNA
Week 8 - Theoretical
Bacterial cloning od DNA (Midterm exam)
Week 9 - Theoretical
Polimerase chain reaction
Week 10 - Theoretical
DNA sequence analysis
Week 11 - Theoretical
RNA isolation
Week 12 - Theoretical
Quality and quantity analysis of RNA
Week 13 - Theoretical
cDNA syntesis
Week 14 - Theoretical
Gen expresion analysis and miRNA Biogenesis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory14103182
Midterm Examination1819
Final Examination1819
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
4
3
OÇ-2
4
4
5
4
2
OÇ-3
4
4
5
4
2
OÇ-4
5
5
5
5
3
OÇ-5
5
4
5
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026