Information Package / Course Catalogue
Recombinant DNA Technology in Agriculture
Course Code: ZBY505
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The goals of this course focus on basic principles and applications of recombinant DNA technology.

Course Content

This course contains all steps of DNA cloning and its applications and also current and the most popular topics in recombinant DNA technology.

Name of Lecturer(s)
Lec. Murat Kemal AVCI
Learning Outcomes
1.Describe current issues about related with Recombinant DNA Technology
2.List all application fields of the technology
3.Explain results of the experiments
4.Distinguish between different methods of the technologies
5.Describe recombinant DNA technology
Recommended or Required Reading
1.Scientific Papers;
2.Current protocols in molecular biology, Edited by Ausubel FM., Brent R., et all., 2003
3.Genes VIII, Lewin B.,Pearson Education International, 2004
4.Molecular biology of the cell, Alberts B., Johnson A., Lewis J., et al., Garland Science, fifth edition, 2008
5.Molecular cloning: a laboratory manual,T. Maniatis, Cold Spring Laboratory Press, 1989
Weekly Detailed Course Contents
Week 1 - Theoretical
Organization of DNA
Week 2 - Theoretical
Genetics of bacteria
Week 3 - Theoretical
Differences between procaryotic and eukaryotic genome
Week 4 - Theoretical
Regulation of gene expression
Week 5 - Theoretical
DNA isolation, restriction enzymes and their application fields
Week 6 - Theoretical
Cloning vectors and features of cloning enzymes
Week 7 - Theoretical
Application: preparation of agar plates and some solutions
Week 8 - Theoretical
Vectors
Week 9 - Theoretical
Gene cloning and gene transfer methods
Week 10 - Theoretical
Application: cloning
Week 11 - Theoretical
Selection of target clones and hybridization techniques
Week 12 - Theoretical
Mutagenesis of cloned genes and analysis of structural and functional changes in the mutant genes
Week 13 - Theoretical
DNA libraries; genomic DNA and cDNA libraries and execution of structure and function analyzes of mutant genes
Week 14 - Theoretical
Litherature analysis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory14103182
Midterm Examination1819
Final Examination1819
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
2
3
4
5
OÇ-2
5
3
4
2
4
OÇ-3
3
4
4
5
3
OÇ-4
5
4
5
4
5
OÇ-5
5
5
4
4
5
Adnan Menderes University - Information Package / Course Catalogue
2026