The aim of this course is to give information about the organization of the laboratory in general, laboratory safety, use of laboratory glassware and chemicals, PCR used in laboratory biotechnological studies, reverse-transchyring PCR (RT-PCR). Restriction enzymes, cDNA synthesis and gene cloning methods, ligation, preparation and transformation of competent bacterial cells, plasmid purification. Molecular markers and analysis methods. Nucleic acid blotting techniques, probe preparation and DNA, RNA labeling methods, southern blotting and DNA analysis, northern blotting and RNA analysis, structure and properties of antibodies, antigen antibody interactions, SDS PAGE, Western blotting, recombinant protein expression, DNA sequencing and analysis methods, microarray and gene expression analysis, genetic transformation techniques and applications, and protein analysis.

Laboratory safety, introduction and use of materials in the laboratory, learning of the chemicals in the laboratory, Use of devices such as PCR, electrophoresis, clevenger, centrifuge.