Information Package / Course Catalogue
Prokaryotic Gene Expression
Course Code: ZBY513
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The objective of this course is to provide an overview of prokaryotic gene structure, function and regulation of the gene expression

Course Content

In this course, it is told about detail information on prokaryotic gene structure and function, knowledge about the mechanisms of DNA synthesis, transcription and translation in prokaryotes, and how the gene expression is regulated in prokaryotes.

Name of Lecturer(s)
Lec. Murat Kemal AVCI
Learning Outcomes
1.Assess prokaryotic gene structure and function.
2.Gain knowledge about the mechanisms of DNA synthesis, transcription and translation in prokaryotes.
3.Tell how the gene expression is regulated in prokaryotes.
4.Genetic Transfer Methods in Bacteria
5.The use of basic plasmids in cloning
Recommended or Required Reading
1.Watson, J.D., et al. Molecular Biology of the Gene. 4. ed. The Benjamin/Cummings Pub. Co., Menlo Park, California, 1988
2.Alberts, B., et al. Molecular Biology of the Cell. 3. ed. Garland Pub., New York, 1994
3.Lewin, B. Genes VII. Oxford University Pres, Oxford, 2000
4.Biology of the Prokaryotes, Lengeler, J., Schlegal, H. (Eds), 1999, Blackwell Publishing, Stutgart, Germany
5.The World of the Cell, Becker, W.M., Kleinsmith, L.J., and Hardin, J.,2000, Addison Wesley Publishing Company, Masachusetts,U.S.A.
6.Wagner, R. Transcription Regulation in Prokaryotes. Oxford University Press, 2000.
Weekly Detailed Course Contents
Week 1 - Theoretical
General view of the prokaryotic cell, cell growth and cell cycle
Week 2 - Theoretical
*DNA Structure and Genetic Information *Supercoiling and histone like proteins *Chromosomes and Other Genetic Elements *Analytic methods in DNA and RNA research
Week 3 - Theoretical
*Mechanisms of DNA replication in prokaryotes *Short overview of eukaryotic DNA replication
Week 4 - Theoretical
*Mechanisms of Transcription in prokaryotes *Short overview of eukaryotic Transcription
Week 5 - Theoretical
Mechanisms of translation in prokaryotes
Week 6 - Theoretical
*Short overview of eukaryotic translation *Genetic code *Protein Folding and Secretion *Molecular chaperones*Expressing Genes in Bacteria
Week 7 - Theoretical
REGULATION *DNA-Binding Proteins and Transcriptional Regulation *Negative control’ versus ‘positive control’ -
Week 8 - Theoretical
Expression Vectors and Regulation of Transcription
Week 9 - Theoretical
*Catabolite Repression *Sensing and Signal Transduction *Quorum Sensing *Heat Shock Proteins *Stringent Response
Week 10 - Theoretical
*Alternative sigma factors *RNA-Based Regulation *Attenuation
Week 11 - Theoretical
Regulation of translation and post-translational modifications
Week 12 - Theoretical
*Methods for Manipulating DNA *Essentials of Molecular Cloning
Week 13 - Theoretical
*Gene Fusions and Reporter Genes *Cloning Vectors, Shuttle Vectors
Week 14 - Theoretical
Molecular Methods for Mutagenesis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory14103182
Midterm Examination1819
Final Examination1819
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
4
5
OÇ-2
5
5
5
5
5
OÇ-3
5
5
5
5
5
OÇ-4
4
4
5
5
5
OÇ-5
3
4
4
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026