Information Package / Course Catalogue
| Course Code | : ZBY541 |
| Course Type | : Area Elective |
| Couse Group | : Second Cycle (Master's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 0 |
| Credit | : 3 |
| Lab | : 2 |
| ECTS | : 8 |
This course covers cell culture techniques used in plant breeding, protoplast isolation in plants, and protoplast hybridizations. Additionally, it includes topics related to determining the ploidy levels of individuals obtained through cell culture in plant breeding.
Cell culture in plants, media used in plant cell culture and their preparation, selection of suitable plant tissue, types of cultures and creation of cultures, protoplast culture, plant tissue selection, protoplast acquisition, methods used in protoplast isolation, protoplast purification, protoplast culture media, somatic hybridization methods, heterokaryon culture, micro callus culture, plant regeneration, flow cytometry
| Prof. Yelda EMEK |
| 1. | Understands the importance of cell culture |
| 2. | Understands cell culture techniques and requirements |
| 3. | Learns somatic hybridization in plant breeding |
| 4. | Learns the methods of using flow cytometry in plant breeding |
| 5. | Understand the importance of somatic hybrids in resistance to stress conditions |
| 1. | Evans, DA, Sharp,WR, Ammirato, PY and Yamada Y. 1983 . Handbook of Plant Cell Culture, New York, Macmillan |
| 2. | Bajaj, YPS.1989. Biotechnology in Agriculture and Forestry: Plant Protoplasts and Genetic Engineering. Berlin:Springer Verlag |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %30 |
| Final Examination | 1 | %70 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 7 | 2 | 126 |
| Laboratory | 14 | 3 | 2 | 70 |
| Midterm Examination | 1 | 3 | 1 | 4 |
| Final Examination | 1 | 4 | 1 | 5 |
| TOTAL WORKLOAD (hours) | 205 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | |
OÇ-1 | 5 | 4 | 4 | 3 | 4 |
OÇ-2 | 4 | 5 | 4 | 3 | 3 |
OÇ-3 | 4 | 5 | 4 | 5 | 4 |
OÇ-4 | 5 | 5 | 4 | 3 | 4 |
OÇ-5 | 5 | 3 | 3 | 4 | 4 |