Information Package / Course Catalogue
Microbiological Counting Methods
Course Code: BİO517
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

This course is designed for Msc students who needs to learn basic counting methods in microbiology. Our aim is to teach the students theoric and practical basis of microbiological counting. Learning of the importance and the number of microorganisms in various environments. Sampling for analyses,cultivation and It is necessary to know and application of numerical microbiological analysis techniques. Learning the distinguishing features of microorganisms between the groups

Course Content

How do microbiological counting, affecting factors ,using areas of counting techniques, conditions that require counting, cultural counting methods, microscobic counting methods, counting techniques based on standard

Name of Lecturer(s)
Prof. Hacı Halil BIYIK
Learning Outcomes
1.To be able to learn the techniques of microbiological counting.
2.To be able to learn the cultural and microscopic counting techniques and to be able to make applications related of these technical3
3.To be able to determine the microbiological count correctly and compare the results with appropriate standards
4.To be able to gain ability for the correct interpretation of the results of microbiological analysis and report writing
5.To be able to learn the possible use of learned methods of counting in practice
6.To be able to procure to evaluate the larned techniques and consumers' complaints
Recommended or Required Reading
1.Velittin GÜRGÜN, A.Kadir HALKMAN, Gıda Teknolojisi Derneği, yayın no:7, San Matbaası, Ankara, 1988
Weekly Detailed Course Contents
Week 1 - Theoretical
General knowledge about microbes, cultural counting technics
Week 1 - Laboratory
Microbiological media and sterilization
Week 2 - Theoretical
Dilutions, colony count and evaluation, MPN Method, microscopic counting techniques
Week 2 - Laboratory
Cultural counts on solid media
Week 3 - Theoretical
Countings with special slides, Spore Counting in Light Microscope
Week 3 - Laboratory
Cultural counts on liquid medium
Week 4 - Theoretical
Counting methods with standarts
Week 4 - Laboratory
Counting of spore and yeast and fungi
Week 5 - Theoretical
Turbidity measurements
Week 5 - Laboratory
General colony count
Week 6 - Theoretical
MacFarland Scale and metabolically counting
Week 6 - Laboratory
Countings with Thoma slides
Week 7 - Theoretical
Methylen Blue Reduction Test
Week 7 - Laboratory
Countings with Howard slides
Week 8 - Theoretical
Reductase test (Midterm exam )
Week 8 - Laboratory
Reductase test
Week 9 - Theoretical
Sampling for analyses
Week 9 - Laboratory
Dilutions Method
Week 10 - Theoretical
Microscopic counting technics
Week 10 - Laboratory
Microscopic counting technics
Week 11 - Theoretical
Indicator microorganisms
Week 11 - Laboratory
MacFarland Scale
Week 12 - Theoretical
Metabolically counting
Week 12 - Laboratory
Total colony count
Week 13 - Theoretical
Anaerobic count
Week 13 - Laboratory
Turbidity Measurements
Week 14 - Theoretical
Microbiological media and sterilization
Week 14 - Laboratory
Pour Plate Technics
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory72228
Assignment2228
Term Project38642
Laboratory70749
Individual Work55550
Midterm Examination110212
Final Examination114216
TOTAL WORKLOAD (hours)205
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
2
OÇ-2
2
OÇ-3
2
OÇ-4
2
OÇ-5
2
OÇ-6
2
Adnan Menderes University - Information Package / Course Catalogue
2026