Information Package / Course Catalogue
Techniques in Molecular Biology I
Course Code: BİO543
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The goal of the course is to teach general molecular biology techniques

Course Content

Genomic and plasmid DNA extraction from different organism and tissues, Nucleic acid detection and separation, mutation detection, PCR, Agarose gel electrophoresis.

Name of Lecturer(s)
Assoc. Prof. Sare İlknur YAVAŞOĞLU
Learning Outcomes
1.To be able to comprehend biomolecule extraction from the cell
2.To be able to comprehend Primer design and PCR
3.To be able to comprehend electrophoresis techniques
4.To be able to comprehend mutation detection
5.Basic knowledge on bioinformatics
Recommended or Required Reading
1.Lecturer notes
2.Sambrook and Russell,(2001) Molecular Clonning, Cold Spring Harbor Laboratory Press, ISBN-0-87969-577-3
3.Temizkan G.,Arda N.(Ed.)(2004),Moleküler Biyolojide Kullanılan Yöntemler,Nobel Kitapevleri,ISBN975-420-347-4
4.Levin B., (2004)Genes VIII, Pearson Education Inc. ISBN-0-19-508956-1
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Structure of biomolecules
Week 2 - Theoretical & Practice
Concentration calculation and buffer solutions
Week 3 - Theoretical & Practice
General purification methods
Week 4 - Theoretical & Practice
DNA extraction from animals tissues
Week 5 - Theoretical & Practice
DNA extraction from plant tissues
Week 6 - Theoretical & Practice
DNA extraction from bacteria
Week 7 - Theoretical & Practice
Plazmid DNA extraction
Week 8 - Theoretical & Practice
Topic repeat (Midterm exam)
Week 9 - Theoretical & Practice
Fungus DNA extraction
Week 10 - Theoretical & Practice
Detection of nucleic acid concentration
Week 11 - Theoretical & Practice
Methods working with proteins
Week 12 - Theoretical & Practice
Agarose gel electrophoresis
Week 13 - Theoretical & Practice
PCR (Polimerase Chain Reaction)
Week 14 - Theoretical & Practice
Bioinformatics, DNA alignment, BLAST, primer design
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Lecture - Practice140228
Assignment218444
Midterm Examination150252
Final Examination150252
TOTAL WORKLOAD (hours)204
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
2
OÇ-2
2
OÇ-3
2
OÇ-4
2
OÇ-5
2
Adnan Menderes University - Information Package / Course Catalogue
2026