Information Package / Course Catalogue
Protein Biochemistry
Course Code: BİO525
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

To learn the structures, functions, synthesis, degradation and analysis methods of proteins, which are the basic organic components of living things.

Course Content

Structure and classification of amino acids, peptides, structure, synthesis and metabolism of proteins and protein determination and analysis methods

Name of Lecturer(s)
Prof. Kubilay METİN
Learning Outcomes
1.Learns and explains the structure of proteins, which are the basic components of living things.
2.Learns and explains the functions of proteins
3.Learns and explains the synthesis of proteins
4.Learns and explains the metabolism of proteins
5.Explains and applies protein determination and analysis methods.
Recommended or Required Reading
1.Biyokimyanın Prensipleri (Lehninger), David L. Nelson & Michael M. Cox ; Nedret Kılıç, Palme Yayıncılık,2005
2.Biyokimya I, II, Engin Gözükara, Nobel Tıp Kitabevi, İstanbul, 1997
3.Analitical Biochemistry, David J. Holme and Hazel Peck, Prentice Hall, 1998.
4.Protein Purification, Robert K. Scopes, Springer-Verlag, 1994
Weekly Detailed Course Contents
Week 1 - Theoretical
Amino acids, amino acids common structural features, classification of amino acids
Week 1 - Preparation Work
Qualitative amino acid determination
Week 2 - Theoretical
Features acid and base of amino acids, titration curve of amino acids
Week 2 - Preparation Work
Quantitative amino acid determination
Week 3 - Theoretical
Methods separate and identify of amino acids
Week 3 - Preparation Work
Separation of amino acids by paper chromatography
Week 4 - Theoretical
Quantify and quality analysis of amino acids
Week 4 - Preparation Work
Separation of amino acids bythin layer chromatography
Week 5 - Theoretical
Peptides, ionization behavior of peptides
Week 5 - Preparation Work
Qualitative protein determination
Week 6 - Theoretical
Biological activity of polypeptides
Week 6 - Preparation Work
Quantitative protein determination (Biüre and Lowry method)
Week 7 - Preparation Work
Quantitative protein determination (Bradford method)
Week 8 - Theoretical
Quantitative protein determination (Lowry method)
Week 9 - Theoretical
Covalent structure of proteins, three-dimensional structure of proteins, primer,
Week 9 - Preparation Work
Protein purification process
Week 10 - Theoretical
Covalent structure of proteins, three-dimensional structure of proteins, primer,
Week 10 - Preparation Work
Protein purification process
Week 11 - Theoretical
Secondary, tertiary and quaternary structure
Week 11 - Preparation Work
Homogenization and centrifuge
Week 12 - Theoretical
Denaturation of proteins,
Week 12 - Preparation Work
Dialysis
Week 13 - Theoretical
Synthesis of proteins
Week 13 - Preparation Work
Gel filtration
Week 14 - Theoretical
Protein metabolism
Week 14 - Preparation Work
SDS-PAGE and protein screening
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice142256
Assignment142142
Individual Work142142
Midterm Examination1325
Final Examination1325
TOTAL WORKLOAD (hours)206
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
2
3
3
2
3
4
2
4
5
OÇ-2
2
3
3
5
1
5
3
2
5
OÇ-3
OÇ-4
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026