Information Package / Course Catalogue
Immunocytochemistry
Course Code: BİO542
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

It aims to give information about immunocytochemical marking methods.

Course Content

It aims to give information about demonstration of cell and tissue antigens by marked antibodies, structure of antibodies, monoclonal ve polyclonal antibodies, direct-indirect methods, immunocytochemical marking methods.

Name of Lecturer(s)
Prof. Yücel KOCA
Learning Outcomes
1.Learns basic knowledge and skills in immunocytochemistry technique.
2.Understands and applies the basic steps of immunohistochemical techniques with all their processes.
3.Interprets and evaluates the data of the immunocytochemistry technique.
4.Immunocytochemistry identifies and can resolve potential problems related to the technique.
5.Relates knowledge on the subject to different fields of biology.
6.Understands and discusses issues related to the subject in scientific platforms.
7.Takes responsibility in theory and practice related to the subject and gains the ability to work individually and in groups.
8.Benefits from relevant databases and has information about current issues.
Recommended or Required Reading
1.- Javois, L.C., Immunocytochemical Methods and Protocols, Humana Press, Second eddition, 460 pages, ISBN: 0-89603-570-0, 1999.
2.- Pound, J.D., Immunochemical Protocols, Humana Press, Second Eddition, 500 pages, ISBN: 0-89603-388-0, 1998.
Weekly Detailed Course Contents
Week 1 - Theoretical
Fixatives and fixation
Week 1 - Preparation Work
Immunocytochemistry definition, importance.
Week 2 - Theoretical
Antibodies, structure of immunoglobulin, antibodies obtaining.
Week 2 - Preparation Work
preparation of sections
Week 3 - Theoretical
Demonstration of cell and tissue antigens by marked antibodies
Week 3 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 4 - Theoretical
Monoclonal ve polyclonal antibodies and characteristics of a good antibody
Week 4 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 5 - Theoretical
Immunocytochemical marking methods; Direct and indirect methods
Week 5 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 6 - Theoretical
Immunocytochemical marking methods; Direct and indirect methods-Contuniation
Week 6 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 7 - Theoretical
Necessary stations for immunocytochemistry; protection of antigens, control of originality of immunocytochemical reactions
Week 7 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 8 - Theoretical
Necessary stations for immunocytochemistry; basic controls ofimmunocytochemistry, method controlling ,control of originality of antiserum (Midterm)
Week 8 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods. (Midterm)
Week 9 - Theoretical
Chromogens and fixation in immunocytochemistry
Week 9 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 10 - Theoretical
Preparation of sections
Week 10 - Theoretical & Practice
Immunocytochemical marking methods; Direct and indirect methods
Week 11 - Theoretical
Preparation of sections
Week 11 - Theoretical & Practice
Immunocytochemical marking methods; Direct and indirect methods
Week 12 - Theoretical
Evaluation of immunocytochemical preparats.
Week 12 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 13 - Theoretical
Problems and solutions being met in immunocytochemistry
Week 13 - Preparation Work
Immunocytochemical marking methods; Direct and indirect methods
Week 14 - Theoretical
General topic review.
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144398
Assignment23312
Reading143384
Midterm Examination1213
Final Examination1213
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
2
4
5
4
2
3
3
4
2
4
OÇ-2
2
5
4
5
4
5
4
5
2
OÇ-3
OÇ-4
OÇ-5
OÇ-6
OÇ-7
OÇ-8
Adnan Menderes University - Information Package / Course Catalogue
2026