Information Package / Course Catalogue
Biochemical Teqniques
Course Code: BİO603
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

Development of biochemical techniques and tool use.

Course Content

Introduction of laboratory instruments and their rules, solution preparation techniques, and the application of many experimental methods used in the field of biochemistry.

Name of Lecturer(s)
Prof. Kubilay METİN
Learning Outcomes
1.Learns and practices working in the laboratory
2.Learns and applies solution preparation techniques
3.Learns and applies laboratory equipment
4.Gains the ability to experiment and designs
5.Analyzes and discusses experimental results
Recommended or Required Reading
1.Analytical Biochemistry David J Holme and Hazel Peck, 2002
2.Principles and Techniques of Practical Biochemistry Edited by K Wilson and J Walker, 2000
Weekly Detailed Course Contents
Week 1 - Theoretical
The solution concentrations
Week 1 - Preparation Work
Presentation tools
Week 2 - Theoretical
Acids, bases, buffer solutions, the calculation of ionic strength
Week 2 - Preparation Work
Preparation of a Solution
Week 3 - Theoretical
Titration curves
Week 3 - Preparation Work
Buffer preperation
Week 4 - Theoretical
Spectrophotometer and spectrophotometric analysis
Week 4 - Preparation Work
Acid titration
Week 5 - Theoretical
Homogenization techniques
Week 5 - Preparation Work
Homogenization of tissue or cell
Week 6 - Theoretical
Centrifugation techniques
Week 6 - Preparation Work
Differential centrifugation of homogenate
Week 7 - Theoretical
Precipitation method
Week 7 - Preparation Work
Ammonium sulfate precipitation
Week 8 - Theoretical & Practice
Protein determination methods
Week 9 - Theoretical
Dialysis
Week 9 - Preparation Work
Dialysis
Week 10 - Theoretical
Enzyme activity determination methods
Week 10 - Preparation Work
Ultrafiltration
Week 11 - Theoretical
Chromatographic techniques
Week 11 - Preparation Work
Gel filtration
Week 12 - Theoretical
Chromatographic techniques
Week 12 - Preparation Work
Ion-exchange chromatography
Week 13 - Theoretical
Chromatographic techniques
Week 13 - Preparation Work
Hydrophobic interaction chromatography
Week 14 - Theoretical
Chromatographic techniques
Week 14 - Preparation Work
Electrophoresis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice142256
Assignment141128
Reading50210
Individual Work142142
Midterm Examination1426
Final Examination1426
TOTAL WORKLOAD (hours)204
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
5
4
3
4
4
3
3
5
4
3
OÇ-2
4
4
4
3
3
4
4
3
4
3
OÇ-3
3
3
3
4
4
3
4
3
4
4
OÇ-4
4
4
4
3
3
4
3
4
3
4
OÇ-5
3
4
3
3
4
3
4
4
3
3
Adnan Menderes University - Information Package / Course Catalogue
2026