Information Package / Course Catalogue
Molecular Techniques in Microbiology
Course Code: MİK649
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 3
Objectives of the Course

The objective of this course is to give information about molecular techniques in microbiology.

Course Content

Purification and seperation of nucleic acids, cutting and joining DNA, Electrophoresis, analysis of gene expression, Northern Blots, RNAse protection assay, Real Time PCR, Reverse transcription PCR, In situ hybridization, Translational analysis, Western Blots, analysis of gene function, manipulating gene expression.

Name of Lecturer(s)
Assoc. Prof. Hafize Tuğba YÜKSEL DOLGUN
Learning Outcomes
1.1. Having information about molecular techniques in microbiology
2.2. Having information about diagnostic ways of molecular techniques in microbiology
3.3. Having information about hybridisation techniques
4.4. Having information about amplification techniques
5.5. Providing ability to use these informations
Recommended or Required Reading
1.Temel Mikrobiyoloji
2.Biyoteknoloji
3.Advances in Veterinary Medicine - Vol 40 Molecular Genetics , Gene Transfer, and Therapy
4.Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Purification of nucleic acids
Week 2 - Theoretical & Practice
Seperation of nucleic acids
Week 3 - Theoretical & Practice
Cutting and joining DNA
Week 4 - Theoretical & Practice
Cutting and joining DNA
Week 5 - Theoretical & Practice
Electrophoresis
Week 6 - Theoretical & Practice
Analysis of gene expression
Week 7 - Theoretical & Practice
Analysis of gene expression
Week 8 - Theoretical & Practice
Discussion
Week 9 - Theoretical & Practice
Analysis of Northern Blots and RNAse protection
Week 10 - Theoretical & Practice
Real time PCR
Week 11 - Theoretical & Practice
Reverse transcription PCR
Week 12 - Theoretical & Practice
In situ hybridization
Week 13 - Theoretical & Practice
Translational analysis
Week 14 - Theoretical & Practice
Analysis of gene function and gene expretion manipulations
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%20
Final Examination1%40
Quiz2%20
Assignment4%20
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Lecture - Practice140228
Assignment4014
Quiz2114
Midterm Examination1516
Final Examination1527
TOTAL WORKLOAD (hours)77
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
4
5
4
5
4
5
4
OÇ-2
5
4
4
4
4
5
4
OÇ-3
4
5
4
5
4
5
4
OÇ-4
4
5
3
5
4
5
5
OÇ-5
5
4
4
5
4
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026