Information Package / Course Catalogue
Methods of Moleculer Diagnosis
Course Code: VPT644
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The use of in situ hybridization and polymerase chain reaction methods in viral, bacterial, parasitic diseases and tumors will be given.

Course Content

The use of in situ hybridization and polymerase chain reaction methods in viral, bacterial, parasitic diseases and tumors will be given.

Name of Lecturer(s)
Assoc. Prof. Emrah İPEK
Learning Outcomes
1.To have knowledge about molecular diagnostic methods
2.To have knowledge about the use of in situ hybridization and polymerase chain reaction methods in viral diseases
3.To have knowledge about the use of in situ hybridization and polymerase chain reaction methods in bacterial diseases
4.To have knowledge about the use of in situ hybridization and polymerase chain reaction methods in parasitic diseases
5.To have knowledge about the use of in situ hybridization and polymerase chain reaction methods in tumors
Recommended or Required Reading
3. Jones, T.C., Hunt, R.D., King, N.W., (1996) Veterinary Patology, Waverly Company, Philadelphia, USA.
4.Thomson, R.G., (1978) General Veterinary Patology, W.B.Saunders Company. Philadelphia, USA.
5.Gavin, M.D.M., Zachary, J. F. (2007) Pathologic Basis of Veterinary Disease, Mosby, London, UK.
6.Robbins, K. C. (1992) Basic Pathology, W.B.Saunders Company, Philadelphia, USA.
7.K.V.F. Jubb, P. C.Kennedy, N. Palmer (1992). Pathology of Domestic Animals volume 1. 4th edition. Academic Pres Inc.
8.K.V.F. Jubb, P. C.Kennedy, N. Palmer (1992). Pathology of Domestic Animals volume 2. 4th edition. Academic Pres Inc.
9.K.V.F. Jubb, P. C.Kennedy, N. Palmer (1992). Pathology of Domestic Animals volume 3. 4th edition. Academic Pres Inc.
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to molecular diagnostic methods
Week 2 - Theoretical
PCR and advanced application techniques
Week 3 - Theoretical
Electrophoresis techniques
Week 4 - Theoretical
Labeling and detection of nucleic acids
Week 5 - Theoretical
Radioactive labeling of DNA ends
Week 6 - Theoretical
DNA hybridization
Week 7 - Theoretical
Pyrosequencing, Midterm
Week 8 - Theoretical
Chain termination method
Week 9 - Theoretical
Chromatography techniques
Week 10 - Theoretical
Southern blotting
Week 11 - Theoretical
Westhern blotting
Week 12 - Theoretical
Northern blotting
Week 13 - Theoretical
Overview
Week 14 - Theoretical
Overview
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Individual Work30927
Midterm Examination1819
Final Examination110111
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
5
5
5
5
5
5
5
5
5
5
OÇ-2
5
5
5
5
5
5
5
5
5
5
OÇ-3
5
5
5
5
5
5
5
5
5
5
OÇ-4
5
5
5
5
5
5
5
5
5
5
OÇ-5
5
5
5
5
5
5
5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026