Information Package / Course Catalogue
Protein Determination Methods
Course Code: VBY643
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

To teach protein detection methods

Course Content

Determination of protein content by biuret method, Lowry protein determination with yönemi, Refractometric analysis, electrophoretic separation of proteins, protein determinations and immunohistochemical immunblot.

Name of Lecturer(s)
Prof. Serap ÜNÜBOL AYPAK
Learning Outcomes
1.Learn the layout of the laboratory
2.Protein determination methods as applied to learn
3.To be able to interpret protein analysis
4.To gain the ability of protein analysis in tissues
5.To learn immunohistochemical methods used in protein determination
Recommended or Required Reading
1.Lippincott’s Illustrated Reviews Biyokimya Seri Editörleri Richard A HARVEY, Pamela C. CHAMPE Biyokimya Çeviri Editörü Doç.Dr. Engin ULUKAYA, Nobel Tıp Kitabevleri 2007
2.Lehninger Biyokimyanın İlkeleri. David L. Nelson Michael M. COX. Çeviri Editörü Prof.Dr. Nedret KILIÇ, Palme Yayıncılık
3.Harper Biyokimya Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Rodwell. Çeviri Editörleri: Nurten DİKMEN, Tuncay ÖZGÜNEN. Nobel Tıp Kitabevleri
Weekly Detailed Course Contents
Week 1 - Theoretical
General information about the methods of protein determination
Week 1 - Practice
Determination of protein content by biuret method
Week 2 - Theoretical
Poteinlerin tayinlerinde kullanılan cihazlar
Week 2 - Practice
Determination of protein content by the method of Lowry
Week 3 - Theoretical
Protein analysis interpretation
Week 3 - Practice
Refractometric analysis
Week 4 - Theoretical
Electrophoretic methods
Week 4 - Practice
Electrophoretic separation of proteins
Week 5 - Theoretical
Protein electrophoresis
Week 5 - Practice
Electrophoretic separation of proteins
Week 6 - Theoretical
SDS-PAGE electrophoresis of proteins
Week 6 - Practice
Electrophoretic separation of proteins
Week 7 - Theoretical
Midterm exam
Week 8 - Theoretical
Immunohistochemical methods for the determination of protein
Week 8 - Practice
Immunohistochemical analyzes of proteins
Week 9 - Theoretical
Immunohistochemical methods for the determination of protein
Week 9 - Practice
Immunohistochemical determination of protein
Week 10 - Theoretical
Determination of protein in tissues
Week 10 - Practice
Determination of protein content in tissues
Week 11 - Theoretical
Methods of determination of albumin
Week 11 - Practice
Methods of determination of albumin
Week 12 - Theoretical
Lipoprotein electrophoresis
Week 12 - Practice
Lipoprotein electrophoresis
Week 13 - Theoretical
Protein analysis to be considered
Week 13 - Practice
Lipoprotein electrophoresis
Week 14 - Theoretical
Student presentations
Week 14 - Practice
Student presentations
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%20
Final Examination1%60
Quiz2%10
Assignment2%10
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Assignment2216
Quiz25011
Midterm Examination1718
Final Examination1718
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
OÇ-1
5
5
5
5
4
5
5
OÇ-2
5
4
3
OÇ-3
5
5
4
5
3
OÇ-4
5
5
4
5
3
OÇ-5
5
5
4
5
3
Adnan Menderes University - Information Package / Course Catalogue
2026