Information Package / Course Catalogue
Immunocytochemistry
Course Code: VHE634
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 5
Objectives of the Course

To teach needed the information for immunocytochemistry

Course Content

Antigens, immunoglobulins, polyclonal and monoclonal antibody, preparation of material and keeping, fixation, embeding and other procedure, enzyme conjugates, immunoenzyme staining tecniques (direct methods and indirect methods), fluorescence conjugates, immunofluoresan staining tecniques, control sections and bacground staining.

Name of Lecturer(s)
Prof. Ülker EREN
Learning Outcomes
1.To be knowledgeable about antigens
2.To be knowledgeable about immunoglobilins
3.To be knowledgeable about antibody of polyclonal and monoclonal
4.Staining methods and staining problems encountered immunocytochemistry and solving these problems
5.To provide solutions to problems
Recommended or Required Reading
1.Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. (1989) Molecular Biology of the Cell, Garland Publishing, Inc. London.
2.Artan E. (1988 ) Histoloji, İstanbul
3.Banks WJ. (1986) Applied Veterinary Histology,Williams& Wilkins, USA.
4.Dellman HD, Brown LM. (1987) Textbook of Veterinary Histology, Lea&Febiger, USA.
5.Gartner LP, Hiatt JL. (1997) Color Textbook of Histology, W.B. Saunders Company,USA.
6.Junqueira LC, Carneiro J. (1983) Basic Histology,The McGraw-Hill Companies,USA
7.Leeson RR, Leeson TS, Paparo AA. (1985) Textbook of Histology, W.B. Saunders Company. USA
8.Ross MH, Reith EJ, Romrell LJ. (1989) Histology. A Text and Atlas, Williams &Wilkins, London
9.Sağlam M, Aştı RN, Özer A. (2001) Genel Histoloji Ders Kitabı, Yorum Matbaacılık, Ankara
Weekly Detailed Course Contents
Week 1 - Theoretical
Antigens
Week 1 - Practice
Antikor titresi ve dilüsyonların hazırlanması
Week 2 - Theoretical
Antigens
Week 2 - Practice
Antibody titer and preparation of dilutions
Week 3 - Theoretical
Immunoglobulins
Week 3 - Practice
Preparation and storage of solutions
Week 4 - Theoretical
Immunoglobulins
Week 4 - Practice
Preparation and storage of solutions
Week 5 - Theoretical
Polyclonal and monoclonal antibody
Week 5 - Practice
Preparation of a suitable medium for stainting
Week 6 - Theoretical
Preparation of material and keeping, fixation
Week 6 - Practice
Preparation of a suitable medium for stainting
Week 7 - Theoretical
Embeding and other procedure
Week 7 - Practice
Preparation of a suitable medium for stainting
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Enzyme conjugates, immunoenzyme staining tecniques (direct methods and indirect methods)
Week 9 - Practice
Direct stainig method
Week 10 - Theoretical
Enzyme conjugates, immunoenzyme staining tecniques (direct methods and indirect methods)
Week 10 - Practice
Applications of complex methods of enzyme-antienzyme
Week 11 - Theoretical
Enzyme conjugates, immunoenzyme staining tecniques (direct methods and indirect methods)
Week 11 - Practice
PAP(Peroxidase-antiperoxidase) staining
Week 12 - Theoretical
Fluorescence conjugates, immunofluoresan staining tecniques
Week 12 - Practice
APAAP stainig
Week 13 - Theoretical
Fluorescence conjugates, immunofluoresan staining tecniques
Week 13 - Practice
Applications of methods Avidin-Biotin
Week 14 - Theoretical
Control sections and bacground staining, Control sections and bacground staining.
Week 14 - Practice
Stainig of immunofluoresan, Stainig of immunofluoresan
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130226
Lecture - Practice130226
Assignment20510
Midterm Examination121122
Final Examination140141
TOTAL WORKLOAD (hours)125
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
4
4
3
OÇ-2
4
4
3
OÇ-3
4
4
3
OÇ-4
4
4
3
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026