Information Package / Course Catalogue
Enzyme Histochemistry
Course Code: VHE641
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 5
Objectives of the Course

The aim of course is to teach enzyme distribution of cells, hyrolytic enzymes, esterases, oxydative enzymes, dehyrogenases and staining methods.

Course Content

Enzyme distribution of cells, hyrolytic enzymes, esterases, oxydative enzymes, dehyrogenases and staining methods.

Name of Lecturer(s)
Lec. Göksel DOĞAN
Prof. Levent KARAGENÇ
Learning Outcomes
1.The student gains knowledge about enzymes.
2.Define the structure and functions of enzymes
3.The student learns enzyme distribution of cells.
4.The student learns staining methods of enzymes.
5.Histochemical determination and evaluation of enzymes
Recommended or Required Reading
1.1. Culling C.F.A.,Allison R.T., Barr W.T. (1985) Cellular PathologyTechnique. London.
2.Luna L.G. (1968) Manual of HistologicStainingMethods of theArmedForcesInstitute of Pathology
3.Sheenan D. C., Hrapchak B.B. (1973) Theoryandpractice of Histotechnology. MosbyCompany
4.Demir R. (2001) Histolojik Boyama Teknikleri. Palme Yayıncılık
Weekly Detailed Course Contents
Week 1 - Theoretical
What is enzyme? The properties of the enzymes.
Week 1 - Practice
To prepare solutions for enzyme stains
Week 2 - Theoretical
Structure of the enzymes
Week 2 - Practice
To prepare solutions for enzyme stains
Week 3 - Theoretical
Classification of enzymes
Week 3 - Practice
Preparation of the enzyme solution required for staining
Week 4 - Theoretical
Enzymes' tasks
Week 4 - Practice
Lead acid phosphatase implementation of the method
Week 5 - Theoretical
The working mechanism of enzymes
Week 5 - Practice
Lead acid phosphatase implementation of the method
Week 6 - Theoretical
To the study of factors affecting enzyme
Week 6 - Practice
Gomori method
Week 7 - Theoretical
Redistribution of the enzyme in cells
Week 7 - Practice
Alpha Naphthyl Acetate Esterase in tissue (ANAE) method
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Hydrolytic enzymes
Week 9 - Practice
Alpha Naphthyl Acetate Esterase in tissue (ANAE) method
Week 10 - Theoretical
Esterases
Week 10 - Practice
Alpha Naphthyl Acetate Esterase (ANAE) method in blood
Week 11 - Theoretical
Oxidative enzymes
Week 11 - Practice
Naphthol AS-D cloraesteraz method for neutrophils
Week 12 - Theoretical
Dehydrogenases
Week 12 - Practice
Naphthol AS-D cloraesteraz method for neutrophils
Week 13 - Theoretical
Staining methods
Week 13 - Practice
For eosinophil peroxidase positive reaction Undritz
Week 14 - Theoretical
Staining metods, Article discussion
Week 14 - Practice
For eosinophil peroxidase positive reaction Undritz, Article presentation
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130113
Lecture - Practice130226
Assignment210122
Midterm Examination125126
Final Examination137138
TOTAL WORKLOAD (hours)125
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
4
4
3
3
OÇ-2
OÇ-3
4
4
3
3
OÇ-4
4
4
3
3
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026