Information Package / Course Catalogue
Establisment of Embryonic Systm Cell Lines in Mammals
Course Code: VHE657
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

To understand mammalian embryo development at pre-implantation stages of development, isolation of mammalian embryos at blastocyst stage of development, isolation and propagation of inner cell mass cells in culture, methods used for the characterization of embryonic stem cell lines and their use in medicine.

Course Content

Development of mammalian embryos at pre-implantation stages of development, establishment of embryonic stem cell lines and their characterization, formation of chimeric using embryonic cells.

Name of Lecturer(s)
Lec. Göksel DOĞAN
Learning Outcomes
1.Gains expert information on mammalian embryo development at pre-implantation stages of development
2.Learns methods used for isolation and propagation of inner cell mass cells in culture
3.Learns methods used for the characterization of embryonic stem cell lines
4.Learns possible uses of embryonic stem cells in medicine
5.Follow current developments in embryonic stem cells in mammalian animals.
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Mammalian embryo development at pre-implantation stages of development
Week 2 - Theoretical
Cell differentiation at blastocyst stage: Establishment of inner cell mass and trophectoderm cells
Week 3 - Theoretical
Isolation of embryos at pre-implantation stages of development
Week 4 - Theoretical
Isolation of inner cell mass cells at the blastocyst stage
Week 5 - Theoretical
Principles of cell culture
Week 6 - Theoretical
Methods and media used for the culture of embryonic stem cells
Week 7 - Theoretical
Growth factors affecting the proliferation of iner cell mass cells
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Transfer of inner cell mass cells to tissue culture plates
Week 10 - Theoretical
Maintenance of inner cell mass cells in tissue culture
Week 11 - Theoretical
Morphological charecterisation and isolation of embryonic stem cell colonies
Week 12 - Theoretical
Characterization of embryonic stem cells
Week 13 - Theoretical
Differentiation of embryonic stem cells
Week 14 - Theoretical
Production of chimeric embryos by transferring embryonic stem cells to a recipient embryo at the blastocyst stage of development
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130113
Reading6016
Midterm Examination120121
Final Examination134135
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-2
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-3
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-4
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026