Information Package / Course Catalogue
Isolation and Culture of Mouse Embryos and Embryo Transfer
Course Code: VHE661
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

Derste yaygın bir model olarak kullanlan farelerde çeşitli gelişim aşamalarında embriyoların izolasyonu, kültürü, embriyo skorlaması ve embriyo transferinde kullanılan tekniklerin öğrenilmesi amaçlanmıştır.

Course Content

Isolation of Mouse embryos at various stages of embryonic development, culture of embryos up to the blastocyst stage of development, scoring of blastocysts, transfer of embryos to recipient pseudo-pregnant mice and examination of the implantation and fetal development.

Name of Lecturer(s)
Lec. Göksel DOĞAN
Learning Outcomes
1.Learns isolation of Mouse embryos at various stages of embryonic development
2.Learns culture of embryos up to the blastocyst stage of development
3.Learns scoring of morula and blastocysts.
4.Learns transfer of embryos to recipient pseudo-pregnant mice and examination of the implantation and fetal development.
5.Evaluates implantation rates after embryo transfer.
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
T: Mammalian embryo development at pre-implantation stages-I
Week 2 - Theoretical
T: Mammalian embryo development at pre-implantation stages-2
Week 3 - Theoretical
T: Embryo metabolism at pre-implantation stages of development
Week 4 - Theoretical
T: Embryo culture media
Week 5 - Theoretical
T: Embryo culture media formulations
Week 6 - Theoretical
T: Embryo culture system-Macromolecules
Week 7 - Theoretical
T: Embryo culture system-pH
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
T: Embryo culture system--Carbondioxide
Week 10 - Theoretical
T: Embryo culture system-Oxygen
Week 11 - Theoretical
T: Embryo culture system-Temperature
Week 12 - Theoretical
T: Importance of quality control in embryo culture
Week 13 - Theoretical
T: New developments in embryo culture
Week 14 - Theoretical
T: Negative effects of embryo culture on the embryo, Negative effects of embryo culture on the embryo
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130226
Seminar1415
Reading4118
Midterm Examination112113
Final Examination122123
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
3
5
4
5
5
3
OÇ-2
3
5
4
5
5
3
OÇ-3
3
5
4
5
5
3
OÇ-4
3
5
4
5
5
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026