
| Course Code | : BYK606 |
| Course Type | : Required |
| Couse Group | : Third Cycle (Doctorate Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 2 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 5 |
It is aimed to give new developments in the field of protein purification in the light of basic techniques.
Structural properties and investigation of proteins, amino acids and chemical proporties, primary structure of proteins: the amino acid sequence, N-terminal and C-terminal, amino acid sequencing / gene relationship, levels of the upper structure of proteins – secondary - tertiary and quaternary structure - the structure - function relationships, proteins and biomembranes: membrane proteins, oxygen-binding proteins: myoglobin and hemoglobin, the protein concentration measurement method I, Bradford methods, the protein concentration measurement method II, Lowry method, BCA method, Biuret method, analytical methods, TCA precipitation, acetone precipitation, immunoprecipitation, concentration of protein solutions, preparative methods, ammonium sulfate precipitation, precipitation with an organic solvent, precipitation with polyethylene glycol, ultrafiltration, dialysis, lyophilization.