Information Package / Course Catalogue
Advanced Laboratory Techniques Used in Food Microbiology
Course Code: VBH622
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The aim of this lecture is to give detailed information about advanced laboratory applications used in food microbiology.

Course Content

ELISA (Enzyme Linked Immunosorbent Assay). RIA (Radio Immun Assay). CFT (Complement Fixation). Hemaglutination, hemaglutination inhibition, immunodiffusion (agar-gel precipitation, immunoelectrophoresis, zone electrophoresis), immunohistochemical methods (direkt and indirekt immunfleurosans), molecular techniques,PCR, Real Time and Multiplex PCR, DNA hybridisation, . Immunofleurosans. pulsed field gel electrophoresis (PFGE), Restriction Fragment Lenght Polymorphism (RFLP)

Name of Lecturer(s)
Prof. Filiz KÖK
Learning Outcomes
1.To gain sufficient knowledge about advanced laboratory techniques
2.To have sufficient knowledge related with ELISA (Enzyme Linked Immunosorbent Assay). RIA (Radio Immun Assay), CFT (Complement Fixation) molecular techniques.
3.To know detailed knowledge about Hemaglutination, hemaglutination inhibition, immunodiffusion (agar-gel precipitation, immunoelectrophoresis, zone electrophoresis), pulsed field gel electrophoresis (PFGE), Restriction Fragment Lenght Polymorphism (RFLP)
4.To have sufficient information related with immunohistochemical methods (direct and indirect immunofluorescence), immunofluorescence.
5.To gain the ability to use the basic knowledge related with advanced molecular applications in food science.
Recommended or Required Reading
1.Koneman's Color Atlas and Textbook of Diagnostic Microbiology
2.Bergey's manual of systematic bacteriology
3.Handbook of Vertebrate Immunology
4.Veterinary Laboratory Medicine
5.The ELISA Guidebook
6.Temel Mikrobiyoloji
7.İmmunoloji
8.Compendium for foods
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to conventional methods in food microbiology
Week 1 - Practice
Presentation of instruments, equipment, and medias used in microbiological analysis
Week 2 - Theoretical
Routine applications in conventional microbiological analysis
Week 2 - Practice
Routine practices
Week 3 - Theoretical
Searching for Salmonella in foods
Week 3 - Practice
Searching for Salmonella in foods
Week 4 - Theoretical
Searching for Campylobacter in foods
Week 4 - Practice
Searching for Campylobacter in foods
Week 5 - Theoretical
Searching for Listeria in foods
Week 5 - Practice
Practice on searching for Listeria in foods
Week 6 - Theoretical
Searching for Yersinia in foods
Week 6 - Practice
Searching for Yersinia in foods
Week 7 - Theoretical
Searching for Pseudomonas,yeast, and mold in foods
Week 7 - Practice
Searching for Pseudomonas, yeast, and mold in foods
Week 8 - Theoretical & Practice
review (Midterm exam)
Week 9 - Theoretical
Searching for coliform, and E. coli in foods
Week 9 - Practice
Searching for coliform, and E. coli in foods
Week 10 - Theoretical
Searching for sulfide reducing in foods
Week 10 - Practice
Searching for sulfide reducing in foods
Week 11 - Theoretical
ELISA test
Week 11 - Practice
Practice on ELISA test
Week 12 - Theoretical
Polymerase Chain Reaction (PCR)
Week 12 - Practice
Practice on PCR
Week 13 - Theoretical
Multiplex PCR method
Week 13 - Practice
Practice on Multiplex PCR method
Week 14 - Theoretical
Immunomanetic Seperation (IMS)
Week 14 - Practice
Practice on Immunomanetic Seperation (IMS)
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Midterm Examination111112
Final Examination120121
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
OÇ-1
5
5
5
5
5
4
5
5
OÇ-2
5
5
5
5
5
5
5
5
OÇ-3
5
5
5
5
5
5
5
5
OÇ-4
5
5
5
4
5
5
5
5
OÇ-5
5
5
5
5
5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026