
| Course Code | : VBH622 |
| Course Type | : Area Elective |
| Couse Group | : Third Cycle (Doctorate Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 1 |
| Prt. | : 2 |
| Credit | : 2 |
| Lab | : 0 |
| ECTS | : 3 |
The aim of this lecture is to give detailed information about advanced laboratory applications used in food microbiology.
ELISA (Enzyme Linked Immunosorbent Assay). RIA (Radio Immun Assay). CFT (Complement Fixation). Hemaglutination, hemaglutination inhibition, immunodiffusion (agar-gel precipitation, immunoelectrophoresis, zone electrophoresis), immunohistochemical methods (direkt and indirekt immunfleurosans), molecular techniques,PCR, Real Time and Multiplex PCR, DNA hybridisation, . Immunofleurosans. pulsed field gel electrophoresis (PFGE), Restriction Fragment Lenght Polymorphism (RFLP)
| Prof. Filiz KÖK |
| 1. | To gain sufficient knowledge about advanced laboratory techniques |
| 2. | To have sufficient knowledge related with ELISA (Enzyme Linked Immunosorbent Assay). RIA (Radio Immun Assay), CFT (Complement Fixation) molecular techniques. |
| 3. | To know detailed knowledge about Hemaglutination, hemaglutination inhibition, immunodiffusion (agar-gel precipitation, immunoelectrophoresis, zone electrophoresis), pulsed field gel electrophoresis (PFGE), Restriction Fragment Lenght Polymorphism (RFLP) |
| 4. | To have sufficient information related with immunohistochemical methods (direct and indirect immunofluorescence), immunofluorescence. |
| 5. | To gain the ability to use the basic knowledge related with advanced molecular applications in food science. |
| 1. | Koneman's Color Atlas and Textbook of Diagnostic Microbiology |
| 2. | Bergey's manual of systematic bacteriology |
| 3. | Handbook of Vertebrate Immunology |
| 4. | Veterinary Laboratory Medicine |
| 5. | The ELISA Guidebook |
| 6. | Temel Mikrobiyoloji |
| 7. | İmmunoloji |
| 8. | Compendium for foods |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %40 |
| Final Examination | 1 | %60 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 0 | 1 | 14 |
| Lecture - Practice | 14 | 0 | 2 | 28 |
| Midterm Examination | 1 | 11 | 1 | 12 |
| Final Examination | 1 | 20 | 1 | 21 |
| TOTAL WORKLOAD (hours) | 75 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | PÇ-12 | PÇ-13 | |
OÇ-1 | 5 | 5 | 5 | 5 | 5 | 4 | 5 | 5 | |||||
OÇ-2 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |||||
OÇ-3 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |||||
OÇ-4 | 5 | 5 | 5 | 4 | 5 | 5 | 5 | 5 | |||||
OÇ-5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |||||