Information Package / Course Catalogue
Lactic Acid Bacteria and Industrial Applications
Course Code: BİO623
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to determine the lactic acid bacteria in fermented foods and It covers the importance of probiotics and their benefits to human health.

Course Content

Importance, ecology, physiology, biodiversity of lactic acid bacteria, phylogenetics and promics, antimicrobials, probiotics and prebiotics, effects on human intestinal microbiome, starter production of cultures and the importance of probiotics in the immune system

Name of Lecturer(s)
Prof. Hacı Halil BIYIK
Learning Outcomes
1.Learning the definition and importance of lactic acid bacteria
2.Learning the ecology and physiology of lactic acid bacteria
3.Biodiversity, phylogenetics, promics of lactic acid bacteria learning
4.Understanding bacteriocin production by lactic acid bacteria
5.Learning about lactic acid bacteria and the human intestinal microbiome
6.Learning about probiotics and prebiotics
7.Lactic acid bacteria and probiotics in fermented foods learning the importance
8.Production of starter lactic acid bacteria and immune response of probiotics learning its importance in the system
9.Isolation and Identification of Lactic Acid Bacteria from Environmental Samples
10.Intracellular pH Determination for the Study of Acid Tolerance of Lactic Acid Bacteria
11.lactic acid bacteria in yogurt production
12.PCR Analysis Methods for Detection and Identification of Beer-Spoilage Lactic Acid Bacteria
Recommended or Required Reading
1.Albuquerque M., Albuquerque C., LeBlanc A.M., LeBlanc J.G., Bedani R. 2021. Lactic Acid Bacteria: A Functional Approach. First Edition, Taylor & Francis Group, 292p.
2.Tamang B., Singh A.K., Pradhan N. 2021. Antibiotic Susceptibility of Lactic Acid Bacteria from Gut of Healthy Individuals. Amazon Digital Services LLC - KDP Print USA, 92p.
3.Chen W. 2019. Lactic Acid Bacteria: Bioengineering and Industrial Applications. First Edition, Springer,Germany, 283p.
4.Vinderola G., Ouwehand A., Salminen S., Wright A.V. 2019. Lactic Acid Bacteria: Microbiological and Functional Aspects. Fifth Edition, CRC Press, USA, 764p.
5.Chen W., Narbad A. 2018. Lactic Acid Bacteria in Foodborne Hazards Reduction: Physiology to Practice. First Edition, Springer, Germany, 310p
6.Urbonaviciene D. 2015. The Use of Lactic Acid Bacteria in the Fermentation of Fruits and Vegetables - Technological and Functional Properties. First Edition, IntechOpen, 305p.
7.Mozzi F., Raya R.R., Vignolo G.M. 2015. Biotechnology of Lactic Acid Bacteria: Novel Applications. Second Edition, John Wiley & Sons, New York, USA, 392p.
Weekly Detailed Course Contents
Week 1 - Theoretical
Definition, importance and overview of lactic acid bacteria
Week 1 - Laboratory
Working principles in the laboratory, the use of chemicals and media preparation techniques
Week 2 - Theoretical
Ecology of lactic acid bacteria
Week 2 - Laboratory
Fermented foods (kefir, cheese, boza, sausage, yogurt, pickles, tarhana, turnip, etc.) lactic acid bacteria isolation-I
Week 3 - Theoretical
Physiology of lactic acid bacteria
Week 3 - Laboratory
Fermented foods (kefir, cheese, boza, sausage, yogurt, pickles, tarhana, turnip, etc.) lactic acid bacteria isolation-II
Week 4 - Theoretical
Fermented foods (kefir, cheese, boza, sausage, yogurt, pickles, tarhana, turnip, etc.) lactic acid bacteria isolation-I
Week 4 - Laboratory
Fermented foods (kefir, cheese, boza, sausage, yogurt, pickles, tarhana, turnip, etc.) lactic acid bacteria isolation-III
Week 5 - Theoretical
Fermented foods (kefir, cheese, boza, sausage, yogurt, pickles, tarhana, turnip, etc.) lactic acid bacteria isolation
Week 5 - Laboratory
The morphology of the isolated bacteria, cultural and biochemical tests analyses
Week 6 - Theoretical
Bacteriocins in lactic acid bacteria
Week 6 - Laboratory
The morphology of the isolated bacteria, cultural and biochemical tests analyses
Week 7 - Theoretical
Lactic acid bacteria and the human intestinal microbiome
Week 7 - Laboratory
The morphology of the isolated bacteria, cultural and biochemical tests analyses
Week 8 - Theoretical
Lactic acid bacteria and the human intestinal microbiome-I, Midterm Exam
Week 8 - Laboratory
The morphology of the isolated bacteria, cultural and biochemical tests analyses
Week 9 - Theoretical
Lactic acid bacteria and the human intestinal microbiome-II
Week 9 - Laboratory
Isolates by molecular methods diagnosing
Week 10 - Theoretical
Properties of probiotics and proteomics
Week 10 - Laboratory
İsolates by molecular methods diagnosing
Week 11 - Theoretical
Mılk product fermentation and lactic acid bacteria
Week 11 - Laboratory
Isolates by molecular methods diagnosing
Week 12 - Theoretical
Meat products fermentation and lactic acid bacteria
Week 12 - Laboratory
Isolates by molecular methods diagnosing
Week 13 - Theoretical
Vegetable and fruit fermentation and lactic acid bacteria
Week 13 - Laboratory
Isolates by molecular methods diagnosing
Week 14 - Theoretical
Production of starter lactic acid bacteria and probiotics
Week 14 - Laboratory
Production of bacteriocin from isolates
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory150230
Assignment200240
Project100220
Laboratory150230
Individual Work200360
Quiz100220
Midterm Examination1011
Final Examination1011
TOTAL WORKLOAD (hours)202
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
OÇ-1
4
3
2
4
4
4
4
5
2
2
2
OÇ-2
3
3
4
4
4
4
3
5
4
1
1
OÇ-3
3
3
3
3
3
4
2
4
3
2
1
OÇ-4
4
3
2
4
4
3
3
3
4
3
3
OÇ-5
3
3
3
3
3
4
4
4
3
3
2
OÇ-6
4
4
4
4
4
4
4
4
4
4
4
OÇ-7
3
3
3
3
2
2
3
4
3
4
2
OÇ-8
4
2
2
2
3
3
3
3
2
3
3
OÇ-9
3
3
3
3
3
4
3
2
3
3
3
OÇ-10
4
4
4
4
4
4
4
4
4
4
4
OÇ-11
1
1
1
1
2
2
1
2
1
1
2
OÇ-12
3
3
3
3
3
3
3
3
2
3
2
Adnan Menderes University - Information Package / Course Catalogue
2026