
| Course Code | : MBTK636 |
| Course Type | : Required |
| Couse Group | : Third Cycle (Doctorate Degree) |
| Education Language | : English |
| Work Placement | : N/A |
| Theory | : 1 |
| Prt. | : 3 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 10 |
The aim of this course is to give ability to use methods for recombinant proteins production and their purification
Laboratory practice will be done and information will be given about genetic design of recombinant proteins, cells used for recombinant protein production, induction methods, cell break, extraction and protein purification methods
| Prof. Özge ÇEVİK |
| 1. | Will have information on gene cloning and recombinant synthesis |
| 2. | Will know and use vectors and cells used for recombinant protein production |
| 3. | Will know and use gene engineering methods and sequence modification with in vitro muthagenesis |
| 4. | Ability to use recombinant protein technology |
| 5. | Will know and use protein purification and analysis methods |
| 1. | Basic Biotechnology by Colin Ratledge and Bjorn Kristiansen, Cambridge, U.K. ; New York : Cambridge University Press, 2006. |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %30 |
| Final Examination | 1 | %70 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 0 | 1 | 14 |
| Lecture - Practice | 14 | 0 | 3 | 42 |
| Assignment | 6 | 0 | 15 | 90 |
| Seminar | 3 | 0 | 6 | 18 |
| Laboratory | 5 | 0 | 4 | 20 |
| Individual Work | 13 | 0 | 3 | 39 |
| Quiz | 6 | 0 | 4 | 24 |
| Midterm Examination | 1 | 0 | 3 | 3 |
| Final Examination | 1 | 0 | 3 | 3 |
| TOTAL WORKLOAD (hours) | 253 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | PÇ-12 | PÇ-13 | |
OÇ-1 | 5 | 5 | 3 | 5 | 5 | 3 | 4 | 4 | 4 | 4 | 3 | 3 | 5 |
OÇ-2 | 5 | 5 | 3 | 5 | 5 | 3 | 4 | 4 | 4 | 4 | 3 | 3 | 5 |
OÇ-3 | 5 | 5 | 3 | 4 | 4 | 3 | 5 | 5 | 5 | 5 | 3 | 3 | 5 |
OÇ-4 | 5 | 5 | 3 | 4 | 4 | 3 | 5 | 5 | 5 | 5 | 3 | 3 | 5 |
OÇ-5 | 5 | 5 | 3 | 4 | 4 | 3 | 5 | 5 | 5 | 5 | 3 | 3 | 5 |