Information Package / Course Catalogue
Recombinant Protein Production and Purification
Course Code: MBTK636
Course Type: Required
Couse Group: Third Cycle (Doctorate Degree)
Education Language: English
Work Placement: N/A
Theory: 1
Prt.: 3
Credit: 3
Lab: 0
ECTS: 10
Objectives of the Course

The aim of this course is to give ability to use methods for recombinant proteins production and their purification

Course Content

Laboratory practice will be done and information will be given about genetic design of recombinant proteins, cells used for recombinant protein production, induction methods, cell break, extraction and protein purification methods

Name of Lecturer(s)
Prof. Özge ÇEVİK
Learning Outcomes
1.Will have information on gene cloning and recombinant synthesis
2.Will know and use vectors and cells used for recombinant protein production
3.Will know and use gene engineering methods and sequence modification with in vitro muthagenesis
4.Ability to use recombinant protein technology
5.Will know and use protein purification and analysis methods
Recommended or Required Reading
1.Basic Biotechnology by Colin Ratledge and Bjorn Kristiansen, Cambridge, U.K. ; New York : Cambridge University Press, 2006.
Weekly Detailed Course Contents
Week 1 - Theoretical
Recombinant proteins and hormones
Week 2 - Theoretical
Vectors used for of recombinant protein production
Week 3 - Theoretical
Restriction enzymes, their use and classification
Week 4 - Practice
DNA and RNA Modification enzymes and their uses, mutagenesis
Week 5 - Practice
Specific and modified primer design and programs
Week 6 - Practice
Gene transfer to E. coli
Week 7 - Practice
Gene transfer to fungi-I
Week 8 - Practice
Gene transfer to fungi-II (Midterm exam)
Week 9 - Practice
Selective media and its preparation
Week 10 - Practice
Induction of recombinant protein synthesis
Week 11 - Practice
Methods for cell disruption and practice
Week 12 - Practice
Methods for protein purification
Week 13 - Practice
Methods for protein purification
Week 14 - Practice
Protein analysis methods
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140342
Assignment601590
Seminar30618
Laboratory50420
Individual Work130339
Quiz60424
Midterm Examination1033
Final Examination1033
TOTAL WORKLOAD (hours)253
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
OÇ-1
5
5
3
5
5
3
4
4
4
4
3
3
5
OÇ-2
5
5
3
5
5
3
4
4
4
4
3
3
5
OÇ-3
5
5
3
4
4
3
5
5
5
5
3
3
5
OÇ-4
5
5
3
4
4
3
5
5
5
5
3
3
5
OÇ-5
5
5
3
4
4
3
5
5
5
5
3
3
5
Adnan Menderes University - Information Package / Course Catalogue
2026