Information Package / Course Catalogue
Biotechnology in Plant Protection
Course Code: BKR222
Course Type: Required
Couse Group: Short Cycle (Associate's Degree)
Education Language: Turkish
Work Placement: Students are required, as part of the course "İMEU201 – Workplace Vocational Training" (30 ECTS) offered in the fourth semester, to undertake and successfully complete workplace vocational training for a minimum of 70 working days at enterprises designated in accordance with the provisions of the Aydın Adnan Menderes University Directive on Workplace Vocational Training.
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 2
Objectives of the Course

The aim of this course is to provide students with the fundamental principles of biotechnology, genetic engineering tools, and application methods in the field of plant protection. Throughout the course, transgenic plant production processes, differences between classical and biotechnological breeding, plant tissue culture techniques (micropropagation, meristem, embryo, anther culture), and environmental, health, and economic risks of GMOs will be covered, aiming for students to critically evaluate biotechnological solutions in agricultural production.

Course Content

The course covers: definition, history, and applications of biotechnology in medicine, industry, environment, and agriculture; comparison of classical and biotechnological methods in plant breeding; basic tools of genetic engineering (restriction enzymes, ligases, vectors); gene transfer methods in plants (Agrobacterium-mediated, biolistics, electroporation); transgenic plant examples (Bt corn, Roundup Ready soy, Golden Rice, drought-tolerant wheat, virus-resistant papaya, blue rose); GMO risks and biosafety; fundamentals of plant tissue culture, sterilization, culture media and plant growth regulators (auxins, cytokinins, gibberellins, abscisic acid, ethylene); micropropagation and meristem culture (virus elimination); somatic embryogenesis, embryo culture and embryo rescue; haploid plant production via anther culture (androgenesis) and ovule/ovary culture (gynogenesis); chromosome doubling (colchicine) and organogenesis.

Name of Lecturer(s)
Learning Outcomes
1.Defines and explains the basic concepts of biotechnology, tools of genetic engineering (restriction enzymes, ligases, vectors), and the concept of transgenic organisms (GMOs).
2.Compares gene transfer methods (Agrobacterium-mediated, biolistics, electroporation) in plants and distinguishes the differences between classical and biotechnological breeding.
3.Evaluates transgenic plant examples (Bt corn, Golden Rice, Roundup Ready soy, etc.) developed for biotic/abiotic stress tolerance, nutritional enhancement, and herbicide tolerance.
4.Explains plant tissue culture techniques (micropropagation, meristem culture, somatic embryogenesis, anther culture, embryo culture) and their applications for disease elimination, propagation, and breeding.
5.Discusses the potential risks and benefits of GMO products on human health, environment, and agricultural economy, and develops a conscious approach to biosafety.
Recommended or Required Reading
1.Course notes of Lecturers
2.Babaoğlu, M., Gürel, E., & Özcan, S. (2001). Plant Biotechnology I: Tissue Culture and Its Applications. Selçuk University Press, Konya.
3.Özcan, S., Gürel, E., & Babaoğlu, M. (2001). Plant Biotechnology II: Genetic Engineering and Its Applications. Selçuk University Press, Konya.
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to Biotechnology: Definition, history, application areas (Medical, Industrial, Environmental, Agricultural)
Week 2 - Theoretical
Plant Breeding Biotechnology: Classical vs. biotechnological breeding, Transgenic (GMO) concept
Week 3 - Theoretical
Fundamentals of Genetic Engineering: DNA, RNA, gene structure, restriction enzymes, ligases, vectors
Week 4 - Theoretical
Gene Transfer Methods: Agrobacterium tumefaciens (Ti-plasmid)-mediated transformation
Week 5 - Theoretical
Gene Transfer Methods: Direct methods (Biolistics-Gene gun, Electroporation, Microinjection)
Week 6 - Theoretical
Transgenic Plant Examples I: Biotic stress (Bt corn, Virus-resistant papaya), Abiotic stress (Drought-tolerant wheat)
Week 7 - Theoretical
Transgenic Plant Examples II: Quality breeding (Golden rice, Blue rose) and Herbicide tolerance (Roundup Ready soy)
Week 8 - Theoretical
Risks and Biosafety of GMOs: Human health (allergy, antibiotic resistance), environment (gene flow, non-target organisms), and economic risks (patents, seed dependence)
Week 9 - Theoretical
Introduction to Plant Tissue Culture: Definition, history, laboratory setup, sterilization methods, contamination sources
Week 10 - Theoretical
Culture Media and Plant Growth Regulators: MS medium, macro/micro elements, vitamins, Auxin, Cytokinin, Gibberellin, ABA, Ethylene (Effects of ratios on callus-root-shoot formation)
Week 11 - Theoretical
Micropropagation and Meristem Culture: Stages, advantages, virus elimination (Potato, strawberry)
Week 12 - Theoretical
Somatic Embryogenesis and Embryo Culture: Direct/indirect embryogenesis, synthetic seeds, embryo rescue (hybridization, early maturing stone fruits)
Week 13 - Theoretical
Haploid Plant Production: Anther culture (Androgenesis), Ovule/Ovary culture (Gynogenesis), factors affecting success (genotype, heat shock, media)
Week 14 - Theoretical
Chromosome Doubling (Colchicine) and Dihaploidization; Organogenesis and General Review (Future of biotechnology in Turkey and the world)
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%10
Quiz1%10
Midterm Examination1%20
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Assignment1213
Quiz1213
Midterm Examination1718
Final Examination1718
TOTAL WORKLOAD (hours)50
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
3
3
3
1
5
2
3
1
3
OÇ-2
3
4
3
1
5
2
3
1
3
OÇ-3
4
5
1
5
2
5
3
4
1
4
OÇ-4
4
3
2
2
4
2
3
2
3
OÇ-5
2
3
5
1
4
5
5
1
5
Adnan Menderes University - Information Package / Course Catalogue
2026