Information Package / Course Catalogue - Adnan Menderes University

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General Biochemistry Laboratory I

Course Code	: BYL355
Course Type	: Required
Couse Group	: First Cycle (Bachelor's Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 0
Prt.	: 2
Credit	: 1
Lab	: 0
ECTS	: 3

Objectives of the Course

To gain the ability to learn and apply biochemical methods.

Course Content

To learn practically the glassware, tools and solution preparation methods used in the laboratory, as well as amino acid, protein and enzyme activity determination methods.

Name of Lecturer(s)

Learning Outcomes

1.	Learns to do research and observation in the laboratory
2.	Gains and applies the ability to use laboratory equipment for the purpose
3.	Learns and applies solution preparation techniques
4.	Learns and applies chromatographic methods
5.	Learns and applies qualitative determination methods of amino acids
6.	Learns and applies quantitative determination methods of amino acids
7.	Learns and applies the working principle of the UV-VIS Spectrophotometry instrument
8.	Learns and applies qualitative and quantitative determination methods of proteins
9.	Learns and applies the determination of enzyme activity
10.	Learns and applies enzyme kinetics

Recommended or Required Reading

1.	Lehninger Principles of Biochemistry
2.	Lecture notes

Weekly Detailed Course Contents

Week 1 - Laboratory

General analysis techniques

Week 2 - Laboratory

General analysis techniques

Week 3 - Laboratory

pH and buffer solutions

Week 4 - Laboratory

Paper chromatography

Week 5 - Laboratory

Qualitative determination of amino acid

Week 6 - Laboratory

Quantitative amino acid determinations

Week 7 - Laboratory

Qualitative determination of protein

Week 8 - Laboratory

Principles of spectrophotometry

Week 9 - Laboratory

Quantitative determination of protein

Week 10 - Laboratory

Quantitative determination of protein

Week 11 - Laboratory

Precipitation of proteins

Week 12 - Laboratory

Enzymes in living tissues

Week 13 - Laboratory

Factors affecting enzyme activity (Temperature-pH)

Week 14 - Laboratory

Factors affecting enzyme activity ( [S] and [E])

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%40
Final Examination	1	%60

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Assignment	2	3	2	10
Laboratory	14	2	2	56
Midterm Examination	1	2	2	4
Final Examination	1	3	2	5
TOTAL WORKLOAD (hours)				75

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7	PÇ-8	PÇ-9	PÇ-10	PÇ-11	PÇ-12	PÇ-13
OÇ-1		4	4				4	4		4
OÇ-2		4	4				4	4		4
OÇ-3		4
OÇ-4	4	4	4		4		4	4
OÇ-5		4	4		4		4
OÇ-6		4	4				4
OÇ-7
OÇ-8
OÇ-9
OÇ-10

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