Information Package / Course Catalogue
Genetic Analysis Technique Based on PCR
Course Code: TBY423
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The aim of this course is to provide information about the usage of polymerase chain reaction (PCR) in genetic assays, to explain different PCR types and to provide theoretical and practical information about PCR based genetic analysis systems to the students.

Course Content

In this course, the history of PCR and how this technique has been developed, PCR chemicals and the preperation of a PCR reactions, amplify a gene region in laboratory, details of different PCR types, explain how PCR technique is used in molecular genetic studies, provide information about the PCR based genetic analysis systems, application of PCRRFLP analysis, PCR errors and their solutions will be disccussed.

Name of Lecturer(s)
Lec. Ferhat KİREMİT
Learning Outcomes
1.Students learn the details of the PCR technique
2.Students learn how the PCR technique is used in molecular genetic studies.
3.Students learn different types of PCR.
4.Students learn PCR based genetic analysis techniques.
5.Students learn the PCR based errors and their corrections.
6.Students learn ISSR analysis.
Recommended or Required Reading
1.The Polymerase Chain Reaction by Kary B. Mullis (Editor), Francois Ferre (Editor), Richard A. Gibbs (Editor), J.D. Watson (Foreword)
Weekly Detailed Course Contents
Week 1 - Theoretical
History of PCR and the development of the PCR techniques.
Week 2 - Theoretical
Different type of DNA molecules, genome structures and and DNA isolation from different tissues
Week 3 - Theoretical
The chemicals used in PCR reactions and the preperation of a PCR reaction
Week 4 - Theoretical
Amplification a gene region
Week 5 - Theoretical
Different PCR types (Gradient PCR, nested PCR, multiplex PCR)
Week 6 - Theoretical
Different PCR types (Hot start PCR, touchdown PCR, reverse transcriptase PCR)
Week 7 - Intermediate Exam
Mid Term Exam
Week 8 - Theoretical
Different PCR types (Allele specific PCR, asymmetric PCR, in situ PCR)
Week 9 - Theoretical
To explain the Quantitative PCR (QPCR) or Real Time PCR (RT PCR) for measuring the quantity of PCR product
Week 10 - Theoretical
PCR errors and solutions of the errors
Week 11 - Theoretical
PCR applications in genetic studies (Molecular identification, Sequence Analyses, Genetic Engineering)
Week 12 - Theoretical
PCR based genetic analysis systems (RAPD, RFLP, AFLP,SSR, etc.)
Week 13 - Laboratory
Application of ISSR analysis in lab
Week 14 - Laboratory
Application of ISSR analysis in lab 2
Week 15 - Theoretical
Discussion on the recent scientific papers about PCR technique.
Week 16 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory122248
Lecture - Practice23210
Midterm Examination1516
Final Examination1516
TOTAL WORKLOAD (hours)70
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
5
4
2
2
1
2
1
5
5
3
5
OÇ-2
4
2
4
2
5
5
5
5
2
1
1
OÇ-3
4
2
1
5
2
3
2
5
5
5
1
OÇ-4
3
2
1
2
4
2
4
2
5
5
2
OÇ-5
3
5
2
2
2
1
2
1
2
1
2
OÇ-6
4
2
5
2
5
2
5
1
2
1
2
Adnan Menderes University - Information Package / Course Catalogue
2026