Information Package / Course Catalogue
DNA and Rna Based Applications
Course Code: TBY416
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 1
ECTS: 4
Objectives of the Course

The focus of Molecular Biology and Biotechnology is DNA and RNA molecules. The aim of this course is to teach students recombinant Technologies, methods and strategies by focusing DNA and RNA, and thanks to these information to provide perspectives and background for applying in agricultural applications.

Course Content

DNA and RNA structure, DNA and RNA synthesis-functions and degradation, Physico-biochemical charachteristics of DNA-RNA and the opportunities provided by these charachteristics for biotechnologic applications, DNA-RNA and molecular biology, DNA-RNA and biotechnology, DNA-RNA and bioethic, PCR, RT-PCR, RT-qPCR, cloning, vectors, restriction enzymes, ligation, transformation, DNA sequence analysis, bioinformatic, electrophoresis systems, DNA-RNA isolation, DGEE, FISH, qualitative analysis, quantitative analysis.

Name of Lecturer(s)
Lec. Murat Kemal AVCI
Learning Outcomes
1.1. DNA and RNA structurs, charachteristics and isolations.
2.2. Associating the structural features of DNA and RNA with molecular biology and biotechnologic methods
3.3. Transformation the DNA-RNA charachteristics from bioinformatic data to wet-lab processes
4.4. Development new strategies in molecular biology and biotechnology.
5.To be able to integrate bioinformatics tools and databases with DNA-RNA applications
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
DNA structure and characteristics
Week 2 - Theoretical & Practice
RNA structure and characteristics
Week 3 - Theoretical & Practice
DNA and RNA isolation
Week 4 - Theoretical & Practice
Qualitative and Quantitative analysis methods
Week 5 - Theoretical & Practice
DNA-RNA concentration determination
Week 6 - Theoretical & Practice
Polymerase Chain Reaction (PCR)
Week 7 - Theoretical & Practice
Vectors, Primer and Prob Design,
Week 8 - Theoretical & Practice
Electrophoresis technics
Week 9 - Theoretical & Practice
PCR-derived methods
Week 10 - Theoretical & Practice
Restriction Enzyme Digestion and Ligation
Week 11 - Theoretical & Practice
Transformation and verification of clones
Week 12 - Theoretical & Practice
Sequence analysis and Bioinformatic
Week 13 - Theoretical & Practice
Denaturing Gradient Gel Electrophoresis (DGGE),
Week 14 - Theoretical & Practice
Fluorescence In Situ Hybridization (FISH)
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice141128
Midterm Examination1718
Final Examination1718
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
3
4
3
3
5
5
3
4
3
2
5
OÇ-2
4
4
4
4
5
4
5
5
3
5
5
OÇ-3
5
5
5
4
5
4
5
5
5
5
5
OÇ-4
4
5
5
5
4
5
5
5
4
4
5
OÇ-5
5
5
5
4
5
4
4
4
3
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026