Information Package / Course Catalogue
Plant Tissue Culture
Course Code: TBY309
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 1
Credit: 3
Lab: 0
ECTS: 4
Objectives of the Course

To provide information about plant cell and tissue culture techniques and to ensure the acquisition of skills for their application.

Course Content

Definition and history of tissue culture, laboratory setup, preparation of culture media, selection of media, plant growth regulators, callus cultures, micropropagation, haploid plant production, protoplast culture and somatic hybridization, somatic embryogenesis, organogenesis, production of secondary products, obtaining virus-free plants, caryopreseration of cultures, tissue culture in plant breeding.

Name of Lecturer(s)
Prof. Yelda EMEK
Learning Outcomes
1.Have learn media using plant tissue culture
2.Have learn plant tissue culture methods
3.To have information about plant tissue culture laboratory organization
4.Apply plant tissue culture methods
5.Have knowledge about the contribution of plant tissue cultures to plant breeding
Recommended or Required Reading
1.Bitki Biyoteknolojisi, Rüştü Hatipoğlu, Adana, 2012.
2.Dixoon, R.A.1985. Plant Cell culture: a practical approach. IRL Press Limited, England, ISBN 0-947946-22-5
3.Bitki Biyoteknolojisi 1. M. Babaoğlu, E. Gürel, S. Özcan. Selçuk Üniversitesi Vakfı Yayınları.
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Tissue culture definition and terminology
Week 2 - Theoretical & Practice
Tissue culture laboratory organization
Week 3 - Theoretical & Practice
Culture media and preparation
Week 4 - Theoretical & Practice
Plant growth regulators
Week 5 - Theoretical & Practice
Organogenesis
Week 6 - Theoretical & Practice
Somatic embryogenesis
Week 7 - Theoretical & Practice
Protoplast culture and somatic hybridization
Week 8 - Theoretical & Practice
Micropropagation
Week 9 - Theoretical & Practice
Embryo culture
Week 10 - Theoretical & Practice
Haploid plant production; anther culture, ovule and ovary cultures
Week 11 - Theoretical & Practice
Cell culture and production of secondary metabolites
Week 12 - Theoretical & Practice
Producing virus-free plants
Week 13 - Theoretical & Practice
In vitro cultures' karyo preservation
Week 14 - Theoretical & Practice
Tissue culture in plant breeding
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Laboratory142142
Midterm Examination1213
Final Examination1213
TOTAL WORKLOAD (hours)104
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
3
1
2
OÇ-2
3
3
4
3
2
3
5
3
2
OÇ-3
2
3
4
3
2
OÇ-4
5
4
3
3
5
3
2
2
1
OÇ-5
4
3
3
3
2
2
3
Adnan Menderes University - Information Package / Course Catalogue
2026