Week 1 - Theoretical
History of molecular biology, discovery of DNA as hereditary material, central dogma
Week 1 - Laboratory
Introduction, Molecular Biology Lab rules, techniques for writing lab notebook and report
Week 1 - Preparation Work
course materials
Week 2 - Theoretical
Nucleic acids I, DNA structure and features
Week 2 - Laboratory
DNA isolation from several samples and determination of quality and quantity via spechtrophotometric analysis
Week 2 - Preparation Work
course materials
Week 3 - Theoretical
Nucleic acids II, RNA structure and features
Week 3 - Laboratory
Explanation of areas of gene cloning, Isolation of genomic DNA from bacteria, and agarose gel electrophoresis
Week 3 - Preparation Work
course materials
Week 4 - Theoretical
The gene, gene structure, prokaryotic and eukaryotic genes
Week 4 - Laboratory
PCR amplification of DNA
Week 4 - Preparation Work
course materials
Week 5 - Theoretical
Structure of chromosomes, Building blocks of chromosomes ( nucleosome and chromatin )
Week 5 - Laboratory
Restriction enzyme digestion of DNA
Week 5 - Preparation Work
course materials
Week 6 - Theoretical
Molecular anatomy of genome, characteristic of genome, gene families
Week 6 - Laboratory
Purification of DNA from agarose gel with several methods
Week 6 - Preparation Work
course materials
Week 7 - Theoretical
Genomic, sequencing and mapping the genome, genome projects
Week 7 - Laboratory
Molecular cloning
Week 7 - Preparation Work
course materials
Week 8 - Preparation Work
course materials
Week 8 - Intermediate Exam
midterm exam
Week 9 - Theoretical
Basic structure and feature of proteins
Week 9 - Laboratory
Transformation of E.coli by electroporation
Week 9 - Preparation Work
course materials
Week 10 - Theoretical
Secondary, tertiary, quaternary protein structures
Week 10 - Laboratory
Selection of organisms containing vector sequences, Screening for clones with desired DNA inserts
Week 10 - Preparation Work
course materials
Week 11 - Theoretical
Proteins had role in nucleic acid metabolisms, and their structures
Week 11 - Laboratory
Southern Blotting
Week 11 - Preparation Work
course materials
Week 12 - Theoretical
DNA replication I ( Mechanism of DNA polymerase, formation of replication fork )
Week 12 - Laboratory
RNA isolation from several samples and determination of quality and quantity via spechtrophotometric analysis
Week 12 - Preparation Work
course materials
Week 13 - Theoretical
DNA replication II ( Key steps form replication initiation to termination )
Week 13 - Laboratory
RNA isolation and making cDNA library
Week 13 - Preparation Work
course materials
Week 14 - Theoretical
Mutation I
Week 14 - Laboratory
Real time PCR analysis
Week 14 - Preparation Work
course materials
Week 15 - Theoretical
Mutation II
Week 15 - Laboratory
Gradient PCR analysis
Week 15 - Preparation Work
course materials
Week 16 - Preparation Work
course materials
Week 16 - Final Exam
final exam
