Week 1 - Theoretical
DNA recombination I ( Molecular analysis of homologous recombination )
Week 1 - Laboratory
In situ hybridization techniques for DNA; Southern and Northern Blotting, DNA-microchips
Week 1 - Preparation Work
course materials
Week 2 - Theoretical
DNA recombination II ( Proteins play role in recombination and their roles, importance of recombination )
Week 2 - Laboratory
Molecular marker techniques
Week 2 - Preparation Work
course materials
Week 3 - Theoretical
DNA repair mechanisms I ( Replication mistakes and repair )
Week 3 - Laboratory
DNA sequencing; Sanger and Maxam-Gilbert techniques and new approaches
Week 3 - Preparation Work
course materials
Week 4 - Theoretical
DNA repair mechanisms II ( Factors of mistakes and repair mechanisms )
Week 4 - Laboratory
DNA sequencing; STR (Short tandem repeats), SNP detection by RFLP (Restriction fragment length polymorphism) method
Week 4 - Preparation Work
course materials
Week 5 - Theoretical
Genetic information flow from DNA to RNA
Week 5 - Laboratory
DNA sequencing; AFLP (Amplified fragment length polymorphism) and ARDRA (Amplified Ribosomal DNA Restriction Analysis)
Week 5 - Preparation Work
course materials
Week 6 - Theoretical
Mechanisms of transcription
Week 6 - Laboratory
Reporter gene construction, reporter assays I
Week 6 - Preparation Work
course materials
Week 7 - Theoretical
RNA editing
Week 7 - Laboratory
Reporter gene construction, reporter assays II
Week 7 - Preparation Work
course materials
Week 8 - Preparation Work
course materials
Week 8 - Intermediate Exam
midterm exam
Week 9 - Theoretical
Genetic code
Week 9 - Laboratory
Protein isolation and purification from bacterial cells
Week 9 - Preparation Work
course materials
Week 10 - Theoretical
Translation I (Information flow from RNA to protein in procaryotes )
Week 10 - Laboratory
SDS-PAGE for protein separation
Week 10 - Preparation Work
course materials
Week 11 - Theoretical
Translation II ( Information flow from RNA to protein in eucaryotes )
Week 11 - Laboratory
Bradford Protein Assay
Week 11 - Preparation Work
course materials
Week 12 - Theoretical
Regulation of gene expression I ( mechanisms in procaryotes )
Week 12 - Preparation Work
course materials
Week 13 - Theoretical
Regulation of gene expression II ( mechanisms in eucaryotes )
Week 13 - Laboratory
Thin Layer Chromatography
Week 13 - Preparation Work
course materials
Week 14 - Theoretical
Experimental methods used in molecular biology
Week 14 - Laboratory
Column Chromatography
Week 14 - Preparation Work
course materials
Week 15 - Theoretical
Current research in molecular biology
Week 15 - Laboratory
Western Blotting
Week 15 - Preparation Work
course materials
Week 16 - Preparation Work
course materials
Week 16 - Final Exam
final exam