Information Package / Course Catalogue
Molecular Biology II
Course Code: BD306
Course Type: Required
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 4
Objectives of the Course

Aim of the course is to teach changable nature of hereditary material, and its repair, molecular basis of gene expression and regulation.

Course Content

Recombination, repair, gene function, gene expression and molecular mechanisms related to the regulation

Name of Lecturer(s)
Learning Outcomes
1. can explain the function, recombination, repair of genes
2.can perform restriction and DNA sequencing analysis.
3.can isolate and purify proteins from organisms
Recommended or Required Reading
1.Watson J. ve diğerleri, 2008. Molecular Biology of the Gene, (7. baskı). San Francisco Pearson yayınları
Weekly Detailed Course Contents
Week 1 - Theoretical
DNA recombination I ( Molecular analysis of homologous recombination )
Week 1 - Laboratory
In situ hybridization techniques for DNA; Southern and Northern Blotting, DNA-microchips
Week 1 - Preparation Work
course materials
Week 2 - Theoretical
DNA recombination II ( Proteins play role in recombination and their roles, importance of recombination )
Week 2 - Laboratory
Molecular marker techniques
Week 2 - Preparation Work
course materials
Week 3 - Theoretical
DNA repair mechanisms I ( Replication mistakes and repair )
Week 3 - Laboratory
DNA sequencing; Sanger and Maxam-Gilbert techniques and new approaches
Week 3 - Preparation Work
course materials
Week 4 - Theoretical
DNA repair mechanisms II ( Factors of mistakes and repair mechanisms )
Week 4 - Laboratory
DNA sequencing; STR (Short tandem repeats), SNP detection by RFLP (Restriction fragment length polymorphism) method
Week 4 - Preparation Work
course materials
Week 5 - Theoretical
Genetic information flow from DNA to RNA
Week 5 - Laboratory
DNA sequencing; AFLP (Amplified fragment length polymorphism) and ARDRA (Amplified Ribosomal DNA Restriction Analysis)
Week 5 - Preparation Work
course materials
Week 6 - Theoretical
Mechanisms of transcription
Week 6 - Laboratory
Reporter gene construction, reporter assays I
Week 6 - Preparation Work
course materials
Week 7 - Theoretical
RNA editing
Week 7 - Laboratory
Reporter gene construction, reporter assays II
Week 7 - Preparation Work
course materials
Week 8 - Preparation Work
course materials
Week 8 - Intermediate Exam
midterm exam
Week 9 - Theoretical
Genetic code
Week 9 - Laboratory
Protein isolation and purification from bacterial cells
Week 9 - Preparation Work
course materials
Week 10 - Theoretical
Translation I (Information flow from RNA to protein in procaryotes )
Week 10 - Laboratory
SDS-PAGE for protein separation
Week 10 - Preparation Work
course materials
Week 11 - Theoretical
Translation II ( Information flow from RNA to protein in eucaryotes )
Week 11 - Laboratory
Bradford Protein Assay
Week 11 - Preparation Work
course materials
Week 12 - Theoretical
Regulation of gene expression I ( mechanisms in procaryotes )
Week 12 - Preparation Work
course materials
Week 13 - Theoretical
Regulation of gene expression II ( mechanisms in eucaryotes )
Week 13 - Laboratory
Thin Layer Chromatography
Week 13 - Preparation Work
course materials
Week 14 - Theoretical
Experimental methods used in molecular biology
Week 14 - Laboratory
Column Chromatography
Week 14 - Preparation Work
course materials
Week 15 - Theoretical
Current research in molecular biology
Week 15 - Laboratory
Western Blotting
Week 15 - Preparation Work
course materials
Week 16 - Preparation Work
course materials
Week 16 - Final Exam
final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141242
Laboratory140228
Midterm Examination110212
Final Examination110212
TOTAL WORKLOAD (hours)94
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
4
OÇ-2
4
OÇ-3
4
Adnan Menderes University - Information Package / Course Catalogue
2026