Information Package / Course Catalogue
Marker Teqnique and Application in Plant Breeding
Course Code: ZBY512
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

Combining the topics of "Plant Breeding" and "Molecular Marker" to give students a vision

Course Content

The definition of molecular markers, techniques used in marker application, PCR-based marker techniques, genetic mapping, marker -based selection

Name of Lecturer(s)
Learning Outcomes
1.Students learn molecular techniques are used in plant breeding
2.Students learn PCR-based molecular techniques
3. Students learn the marker -based selection methods
4.Have knowledge about shortening process in plant breeding by using marker techniques
5.Prepare project on molecular marker techniques used in plant breeding
Recommended or Required Reading
1.Plant Molecular Breeding (Editor: H. John Newbury)(2003)
2.Genome Mapping and Molecular Breeding in Plants(Fruits and Nuts) (Editor:Chittaranjan Kole)(2007)
3.Genome Mapping and Molecular Breeding in Plants(Vegetables) (Editor:Chittaranjan Kole)(2007)
Weekly Detailed Course Contents
Week 1 - Theoretical
Plant Breeding: Definition, Breeding methods, agricultural importance and Objectives
Week 1 - Practice
Laboratory introduction
Week 2 - Theoretical
Molecular Markers: Definition, Types, importance of molecular markers in breeding activities
Week 2 - Practice
Laboratory safety
Week 3 - Theoretical
Use of Molecular Markers in Plant Breeding: Identification of genetic resources, conservation and phylogenetic analysis
Week 3 - Practice
DNA isolation methods
Week 4 - Theoretical
Techniques used in the Marker Practice Approaches: nucleic acid isolation, Polymerase Chain Reaction
Week 4 - Practice
RNA isolation methods
Week 5 - Theoretical
The technical approach used in Marker Application: PAGE, capillary electrophoresis, sequence analysis
Week 5 - Practice
Gel preparation
Week 6 - Theoretical
AFLP (Amplified Fragment Length Polymorphism) principles and methods of application
Week 6 - Practice
Introduction of markers used in molecular breeding
Week 7 - Theoretical
SSR (Simple Sequence Repeats, microsatellites) principles and methods of application
Week 7 - Practice
PCR product preparation
Week 8 - Theoretical
NA, Marker etc. adjustment of concentrations
Week 8 - Practice
SSR detection methods
Week 9 - Theoretical
Gel imaging systems
Week 9 - Practice
SNP (Single Nucleotide Polymorphism in) the principles and methods of application
Week 10 - Theoretical
Application of AFLP technique
Week 10 - Practice
Interpretation of gel results
Week 11 - Theoretical
SNP detection methods
Week 11 - Practice
Implementation of ISSR technique
Week 12 - Theoretical
Genetic mapping
Week 12 - Practice
Sequencing techniques
Week 13 - Theoretical
Marker-based selection (Marker Assisted Selection MAS)
Week 13 - Practice
Gel reading and scoring
Week 14 - Theoretical
Mapping based cloning (Map-Based Cloning)
Week 14 - Practice
Interpretation of gel results
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory143270
Lecture - Practice143270
Midterm Examination1415
Final Examination1415
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
4
4
4
5
5
5
5
OÇ-2
3
4
4
4
4
4
4
OÇ-3
4
4
4
4
4
5
5
OÇ-4
4
5
5
5
5
5
5
OÇ-5
4
4
5
4
4
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026