Information Package / Course Catalogue
Tissue Culture Applications in Horticulture
Course Code: ZBB506
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

The aim of this course is to acquire information about tissue culture applications in horticultural crops to the students at the Science Institute.

Course Content

Importance of tissue culture, laboratory techniques, organogenesis, somatic embryogenesis, disease-free palnt production, micropropagation, embryo culture, anther and pollen culture, protoplast culture and somatic hybridisation, secondary metabolite ğrpduction, germplasm conservation, somaclonal variation in horticultural crops.

Name of Lecturer(s)
Learning Outcomes
1.To be able to perform tissue culture, pratical applications
2.To be able to understand and interpret the problems encountered in tissue culture
3.To be able to examine and evaluate developments
4.To be able to use their acquired knowledge in pratic
5.To be able to reach information sources
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Basic tissue culture laboratory techniques Introduction of laboratory
Week 2 - Theoretical & Practice
Miicropropagation Nutrient media introduction
Week 3 - Theoretical & Practice
Organogenesis and somatic embryogenesis Calculation of ingredients
Week 4 - Theoretical & Practice
Embryo culture Sterilisation
Week 5 - Theoretical & Practice
Disease-free plant production Preparation of nutrient stocks
Week 6 - Theoretical & Practice
Callus culture Preparation of nutrient medis
Week 7 - Theoretical & Practice
In vitro pollination and fertilisation (Midterm exam) Organ culture
Week 8 - Theoretical & Practice
Haploid plant production
Week 9 - Theoretical & Practice
Meristem culture Cell culture
Week 10 - Theoretical & Practice
In vitro pollination Germplasm conservation
Week 11 - Theoretical & Practice
Embryo culture Secondary metabolite producion and somaclonal variation
Week 12 - Theoretical & Practice
Secondary metabolite producion and somaclonal variation Protoplasm culture and somatic hybridisation
Week 13 - Theoretical & Practice
Sub-culture and observation-2 Tissue culture applications in horticultural crops-1
Week 14 - Theoretical & Practice
Sub-culture and observation-3 Tissue culture applications in horticultural crops-2
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory143270
Lecture - Practice143270
Midterm Examination1617
Final Examination1617
TOTAL WORKLOAD (hours)154
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
4
4
5
5
5
4
5
OÇ-2
5
4
4
4
5
4
4
OÇ-3
5
4
5
4
5
5
4
OÇ-4
4
5
5
5
4
4
4
OÇ-5
5
5
5
5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026