Information Package / Course Catalogue
Serology of Plant Viruses
Course Code: ZBK532
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

Understanding of the antigenic properties of plant viruses Intended to be used in the diagnosis of the antigenic properties of viruses.

Course Content

The purpose of this course is to explain the antigenic properties of plant viruses. Because of that ımmune response, ımmunology, ımmunoglobulin and antigenic reaction, antibody molecules, antibody response, antigens and antigenic determinants, immunizing anımals are described. Moreover polyclonal and monoclonal (hibridoma tecniques ) antibodies, virus purification, preparation of antisera and purification of antibodies , antigen-antibody ınteraction, serological techniques (presipitation, diffusion, immunoblotting, immunoelectrophoresis, immunoelectromicroscopy ,ELISA)and Immunochemical studies of plant viruses are clarified.

Name of Lecturer(s)
Learning Outcomes
1.Understanding of the immune response
2.Learning of the antigenic determinants …
3.3. Understanding of antigen-antibody interaction …
4.Application of serological methods …
5.
Recommended or Required Reading
1.Van Regenmortel M.H.V. 1982 Serology and immunochemistry of Plant Viruses, Academic Pres New York 267p
2.Harlow E. And Lane D. 1988 Antibodies A Laboratory Manual CSH Lab.
3.Hill S.A.1984 Methods in Plant Virology Blackwell S.P. London 165p
Weekly Detailed Course Contents
Week 1 - Theoretical
Immune response , Immunology
Week 2 - Theoretical
Immunoglobulin and antigenic reaction
Week 3 - Theoretical
Antibody molecules
Week 4 - Theoretical
Antibody- antigen interaction
Week 5 - Theoretical
Antibody response
Week 6 - Theoretical
Immunızations, pure antigens
Week 7 - Theoretical
Immunizing animals
Week 8 - Theoretical
Polyclonal and Monoclonal antibodies
Week 9 - Theoretical
Virus purification
Week 10 - Theoretical
Preparation of antisera and purification of antibodies
Week 11 - Theoretical
Serolojik teknikler: presipitation, diffusion, immunoelectrophoresis, immunoelectromicroscopy
Week 12 - Theoretical
Immunoblotting, ELISA
Week 13 - Theoretical
Immunoblotting, ELISA
Week 14 - Theoretical
Immunochemical studies of plant viruses
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice143270
Midterm Examination110111
Final Examination112113
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
OÇ-1
3
4
4
4
5
3
OÇ-2
4
4
5
3
5
4
OÇ-3
3
4
5
4
5
4
OÇ-4
5
5
5
5
5
5
OÇ-5
4
3
5
4
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026