Information Package / Course Catalogue
Molecular Identification For Plant-Associated Fungi and Phylogenetic Analysis
Course Code: ZBK512
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

The aim of this course is to to provide knowledge of the molecular techniques used in the taxonomic investigation and identification of plant-associated pathogenic and beneficial fungi.

Course Content

This course provides fundamental knowledge on molecular techniques used in fungal taxonomy, recent molecular advances in the identification of biological control agents, the application of molecular methods for the detection and diagnosis of plant pathogens, and phylogenetic analysis.

Name of Lecturer(s)
Learning Outcomes
1.Acquires detailed knowledge of molecular techniques used in Mycology
2.To provide detailed knowledge of molecular approaches used in identification of plant fungal pathogens and beneficial microorganisms.
3.Acquires practical skills in molecular analysis of fungi, including DNA extraction, PCR, and interpretation of sequencing results.
4.To provide detailed knowledge of molecular responses in host plant–fungal pathogen interactions
5.To enable understanding of fungal evolutionary processes through phylogenetic analysis
Recommended or Required Reading
1.Gherbawy Y., Voigt K. (2010). Molecular Identification of Fungi. Springer Pres, 522 p.
2.Cumagun, C. J. (Ed.). (2012). Plant Pathology. InTech. 376 p.
3.White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR Protocols: a guide to methods and applications. New York: Academic Press; p. 315–322.
4.Gao, C., & Cai, L. (2022). Fungal Taxonomy, Phylogeny, and Ecology: A Themed Issue Dedicated to Academician Wen-Ying Zhuang. Journal of Fungi, 506 p.
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Sampling methods in molecular mycology
Week 2 - Theoretical & Practice
Factors limiting of molecular techniques in fungal diagnostics
Week 3 - Theoretical & Practice
Factors limiting of molecular techniques in fungal diagnostics
Week 4 - Theoretical & Practice
Genomic DNA isolation and extraction methods from fungi
Week 5 - Theoretical & Practice
Fundamentals, steps, and applications of Polymerase Chain Reaction (PCR)
Week 6 - Theoretical & Practice
Electrophoresis techniques and agarose gel electrophoresis
Week 7 - Theoretical & Practice
Sequence analysis software: installation, configuration, and applications
Week 8 - Theoretical & Practice
Using the NCBI GenBank database for sequence submission and obtaining accession numbers
Week 9 - Theoretical & Practice
Introduction to fungal population genetics and basic molecular markers
Week 10 - Theoretical & Practice
Phylogenetic tree structures and representation of evolutionary relationships
Week 11 - Theoretical & Practice
Software and tools for phylogenetic analysis
Week 12 - Theoretical & Practice
Phylogenetic analysis of multiple gene datasets
Week 13 - Theoretical & Practice
Phylogenetic tree construction and interpretation using MEGA X software
Week 14 - Theoretical & Practice
General evaluation and future perspectives on molecular studies in mycology
Assessment Methods and Criteria
Type of AssessmentCountPercent
Presentation1%10
Quiz1%10
Midterm Examination1%10
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice142256
Quiz1415
Midterm Examination112113
Final Examination119120
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
OÇ-1
5
2
OÇ-2
3
OÇ-3
5
3
5
5
OÇ-4
3
OÇ-5
4
2
4
Adnan Menderes University - Information Package / Course Catalogue
2026