Information Package / Course Catalogue
Tissue Culture Applications in Horticulture
Course Code: ZBB506
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to acquire information about tissue culture applications in horticultural crops to the students at the Science Institute.

Course Content

Importance of tissue culture, laboratory techniques, organogenesis, somatic embryogenesis, disease-free palnt production, micropropagation, embryo culture, anther and pollen culture, protoplast culture and somatic hybridisation, secondary metabolite ğrpduction, germplasm conservation, somaclonal variation in horticultural crops.

Name of Lecturer(s)
Prof. Gonca GÜNVER DALKILIÇ
Learning Outcomes
1.To be able to perform tissue culture, pratical applications
2.To be able to understand and interpret the problems encountered in tissue culture
3.To be able to examine and evaluate developments
4.To be able to use their acquired knowledge in pratic
5.To be able to reach information sources
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Basic tissue culture laboratory techniques
Week 1 - Practice
Introduction of laboratory
Week 2 - Theoretical
Miicropropagation
Week 2 - Practice
Nutrient media introduction
Week 3 - Theoretical
Organogenesis and somatic embryogenesis
Week 3 - Practice
Calculation of ingredients
Week 4 - Theoretical
Embryo culture
Week 4 - Practice
Sterilisation
Week 5 - Theoretical
Disease-free plant production
Week 5 - Practice
Preparation of nutrient stocks
Week 6 - Theoretical
Callus culture
Week 6 - Practice
Preparation of nutrient medis
Week 7 - Theoretical
In vitro pollination and fertilisation
Week 7 - Practice
Organ culture
Week 8 - Intermediate Exam
Midterm Exam
Week 9 - Theoretical
Haploid plant production
Week 9 - Practice
Meristem culture
Week 10 - Theoretical
Cell culture
Week 10 - Practice
In vitro pollination
Week 11 - Theoretical
Germplasm conservation
Week 11 - Practice
Embryo culture
Week 12 - Theoretical
Secondary metabolite producion and somaclonal variation
Week 12 - Practice
Sub-culture and observation-1
Week 13 - Theoretical
Protoplasm culture and somatic hybridisation
Week 13 - Practice
Sub-culture and observation-2
Week 14 - Theoretical
Tissue culture applications in horticultural crops-1
Week 14 - Practice
Sub-culture and observation-3
Week 15 - Theoretical
Tissue culture applications in horticultural crops-2
Week 15 - Practice
Sub-culture and observation-4
Week 16 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144284
Lecture - Practice145298
Midterm Examination1617
Final Examination1617
TOTAL WORKLOAD (hours)196
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
3
3
3
3
3
3
3
3
3
3
OÇ-2
3
3
3
3
3
3
3
3
3
3
OÇ-3
3
3
3
3
3
3
3
3
3
3
OÇ-4
4
4
4
4
4
4
4
4
4
4
OÇ-5
4
4
4
4
4
4
4
4
4
4
Adnan Menderes University - Information Package / Course Catalogue