Information Package / Course Catalogue
Enzyme Biochemistry
Course Code: BİO523
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to explain the properties of the enzymes and principles of catalytic mechanisms

Course Content

Enzyme structure, classification, catalytic mechanisms, kinetics, activation and inhibition

Name of Lecturer(s)
Learning Outcomes
1.To be able to learn the Enzyme structure
2.To be able to classify the enzymes
3.To be able to learn the mechanisms of catalysis
4.To be able to get the Enzyme kinetics
5.To be able to analyze Activation and inhibition of enzymes
6.To be able to get Information about the measurement of enzyme activity and kinetic characterization
Recommended or Required Reading
1.Biyokimyanın Prensipleri (Lehninger), David L. Nelson & Michael M. Cox ; Nedret Kılıç, Palme Yayıncılık,2005
2.Biyokimya I, II, Engin Gözükara, Nobel Tıp Kitabevi, İstanbul, 1997
3.Enzymes, A Practical Introduction to Structure, Mechanism and Data Analysis, Robert A. Copeland, A. John Wiley and Sons Inc. Publication, 2000
4.Enzyme Chemistry Impact and applications, C. J. Suckling, C. L. Gibson, A. R. Pitt, Blackie Academic and Professional, 1998.
Weekly Detailed Course Contents
Week 1 - Theoretical
Common features of enzymes
Week 1 - Preparation Work
Enzyme purification
Week 2 - Theoretical
Classification of enzymes
Week 2 - Preparation Work
Enzyme purification
Week 3 - Theoretical
Enzymatic catalyst mechanism
Week 3 - Preparation Work
Enzyme purification
Week 4 - Theoretical
Enzyme kinetics
Week 4 - Preparation Work
Enzyme activity assays
Week 5 - Theoretical
Factors influencing enzyme activity
Week 5 - Preparation Work
Factors influencing enzyme activity (pH and temperature)
Week 6 - Theoretical
Inhibition of enzymes
Week 6 - Preparation Work
Factors influencing enzyme activity (pH and temperature stability)
Week 7 - Theoretical
Allosteric enzymes
Week 7 - Preparation Work
Factors influencing enzyme activity (Substrate concentration)
Week 8 - Theoretical
Allosteric enzymes
Week 8 - Preparation Work
Factors influencing enzyme activity (Substrate concentration)
Week 9 - Intermediate Exam
Mid exam
Week 10 - Theoretical
Enzyme specificity
Week 10 - Preparation Work
Enzyme specificity
Week 11 - Theoretical
Enzymatic catalyst mechanism
Week 11 - Preparation Work
Enzymatic catalyst mechanism
Week 12 - Theoretical
Regulation of enzyme activity
Week 12 - Preparation Work
Regulation of enzyme activity
Week 13 - Theoretical
Determination of enzyme activity, spectrofotometric applications.
Week 13 - Preparation Work
Determination of enzyme activity, spectrofotometric applications.
Week 14 - Theoretical
Structure and function of coenzymes.
Week 14 - Preparation Work
Structure and function of coenzymes.
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory150230
Lecture - Practice150230
Assignment70535
Project70535
Laboratory100550
Reading4028
Quiz4028
Midterm Examination1022
Final Examination1022
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
4
4
4
4
4
4
4
4
4
4
OÇ-2
3
3
3
3
3
3
3
3
3
3
OÇ-3
4
4
4
4
4
4
4
4
4
4
OÇ-4
3
3
3
3
3
3
3
3
3
3
OÇ-5
4
4
4
4
4
4
4
4
4
4
OÇ-6
3
3
3
3
3
3
3
3
3
3
Adnan Menderes University - Information Package / Course Catalogue